Expression and characterization of MAP kinases in bacteria

Charles J. Heise, Melanie H. Cobb

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Mitogen-activated protein kinases (MAPKs) are common signal transducers in all eukaryotic organisms. MAPKs are activated by protein kinase cascades consisting of MAPK kinases (MAP2Ks) and MAPK kinase kinases (MAP3Ks). Extracellular-signal regulated kinases 1 and 2 (ERK1/2) are the best characterized MAPKs. Like other MAPKs their activity is regulated by dual phosphorylation as well as dephosphorylation by a host of phosphoprotein phosphatases. The ability to phosphorylate or thiophosphorylate ERK2 in vitro, as described here, is valuable for use in downstream applications designed to investigate MAPK signaling networks.

Original languageEnglish (US)
Pages (from-to)209-212
Number of pages4
JournalMethods
Volume40
Issue number3
DOIs
StatePublished - Nov 2006

Keywords

  • ERK2
  • In vitro activation
  • Phosphorylation
  • Protein kinase

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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