Expression and mutation of the tumor suppressor gene p53 in AIDS- associated Kaposi's sarcoma

Jian J. Li, Yao Q. Huang, Clay J. Cockerell, Wei G. Zhang, Alexander Nicolaides, Alvin E. Friedman-Kien

Research output: Contribution to journalArticle

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Abstract

Alteration of the p53 gene is the most frequent event reported in human cancer, and p53 mutations have been observed in various neoplasms, including certain forms of skin cancer. Therefore, we postulated that p53 may also be involved in Kaposi's sarcoma associated with AIDS (AIDS-KS). Expression of the p53 gene was examined in freshly isolated tumor biopsy specimens from 15 patients with AIDS-KS. p53 mRNA was detected by reverse transcriptase- polymerase chain reaction (RT-PCR) in both the AIDS-KS tumors and in normal skin control samples. p53 protein was detected in 4 of the 15 AIDS-KS specimens by immunohistochemical staining. Single-strand conformation polymorphism analysis PCR-products (PCR-SSCP) was used for detection of mutations of the p53 gene. One of the p53 positive AIDS-KS samples showed mobilized shifts in exon 6 suggestive of a mutation. Sequencing data showed the mutation to be located in codon 210. We examined other mechanisms that could stabilize p53 protein. SV40 large T antigen and adenovirus E1B protein were not found in the AIDS-KS specimens. MDM2, a p53-binding protein, was also detected in five of the AIDS-KS specimens, two of which also contained p53-positive cells. These observations suggest that the tumor suppressor gene p53 may be involved in the pathogenesis of AIDS-KS.

Original languageEnglish (US)
Pages (from-to)373-378
Number of pages6
JournalAmerican Journal of Dermatopathology
Volume19
Issue number4
DOIs
StatePublished - 1997

Fingerprint

Kaposi's Sarcoma
Tumor Suppressor Genes
Acquired Immunodeficiency Syndrome
Mutation
p53 Genes
Adenovirus E1B Proteins
Neoplasms
Polyomavirus Transforming Antigens
Single-Stranded Conformational Polymorphism
Polymerase Chain Reaction
Viral Tumor Antigens
Skin Neoplasms
Reverse Transcriptase Polymerase Chain Reaction
Codon
Exons
Carrier Proteins
Proteins
Staining and Labeling
Biopsy
Messenger RNA

Keywords

  • Kaposi's sarcoma
  • MDM2
  • p53

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Dermatology

Cite this

Expression and mutation of the tumor suppressor gene p53 in AIDS- associated Kaposi's sarcoma. / Li, Jian J.; Huang, Yao Q.; Cockerell, Clay J.; Zhang, Wei G.; Nicolaides, Alexander; Friedman-Kien, Alvin E.

In: American Journal of Dermatopathology, Vol. 19, No. 4, 1997, p. 373-378.

Research output: Contribution to journalArticle

Li, Jian J. ; Huang, Yao Q. ; Cockerell, Clay J. ; Zhang, Wei G. ; Nicolaides, Alexander ; Friedman-Kien, Alvin E. / Expression and mutation of the tumor suppressor gene p53 in AIDS- associated Kaposi's sarcoma. In: American Journal of Dermatopathology. 1997 ; Vol. 19, No. 4. pp. 373-378.
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