Expression and targeting of a 47 kDa integral peroxisomal membrane protein of Candida boidinii in wild type and a peroxisome-deficient mutant of Hansenula polymorpha

G. J. Sulter, H. R. Waterham, E. G. Vrieling, J. M. Goodman, W. Harder, M. Veenhius

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A 47 kDa integral peroxisomal membrane protein (PMP47) of Candida boidinii was expressed in wild type (WT) and a temperature-sensitive (Ts6) peroxisome-deficient (per) mutant of Hansenula polymorpha. The subcellular location of PMP47 appeared to be dependent on the level of expression. At low expression levels PMP47 was sorted to the peroxisomal membrane; however, in Ts6 cells grown at restrictive temperatures (which lack intact peroxisomes) PMP47 was solely located in small cytosolic aggregates, together with homologous H. polymorpha PMP's. At enhanced expression levels, however, part of the protein also became incorporated into mitochondria, both in transformed WT and Ts6 cells.

Original languageEnglish (US)
Pages (from-to)211-216
Number of pages6
JournalFEBS Letters
Volume315
Issue number3
DOIs
StatePublished - Jan 11 1993

Keywords

  • Candida boidinii
  • Hansenula polymorpha
  • Peroxisomal membrane protein
  • Peroxisome
  • Peroxisome-deficient mutant
  • Protein targeting
  • Yeast

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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