Expression cloning of a diphtheria toxin receptor: Identity with a heparin-binding EGF-like growth factor precursor

Joseph G. Naglich, James E. Metherall, David W. Russell, Leon Eidels

Research output: Contribution to journalArticlepeer-review

447 Scopus citations

Abstract

A monkey cDNA (pDTS) encoding a diphtheria toxin (DT) sensitivity determinant was isolated by expression cloning in mouse L-M cells. Mouse cells are naturally resistant to DT, because they lack functional cell surface receptors for the toxin. Unlike wild-type L-M cells, pDTS-transfected mouse cells are extremely toxin sensitive and specifically bind radioiodinated DT. Intoxication of the transfected cells requires receptor-mediated endocytosis of the bound toxin. The cDNA is predicted to encode an integral membrane protein that is identical to the precursor of a heparin-binding EGF-like growth factor. The DT sensitivity protein is thus a growth factor precursor that DT exploits as a receptor.

Original languageEnglish (US)
Pages (from-to)1051-1061
Number of pages11
JournalCell
Volume69
Issue number6
DOIs
StatePublished - Jun 12 1992

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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