Expression cloning of SR-BI, a CD36-related class B scavenger receptor

Susan L. Acton, Philipp E. Scherer, Harvey F. Lodish, Monty Krieger

Research output: Contribution to journalArticle

679 Citations (Scopus)

Abstract

Scavenger receptors are integral membrane proteins that mediate the endocytosis of modified lipoproteins. The first of these to be purified and cloned were the type I and II macrophage scavenger receptors (SR-AI and SR- AII; class A scavenger receptors). Subsequently, the cell surface protein CD36 was shown to bind oxidized low density lipoprotein (oxidized LDL). From a Chinese hamster ovary (CHO) cell variant we have cloned by expression the cDNA for a new member of the CD36 family of membrane proteins, SR-BI, whose predicted protein sequence of 509 amino acids is ~30% identical to those of the four previously identified family members. Both SR-BI and CD36 displayed high affinity binding for acetylated LDL with an apparent dissociation constant on the order of ~5 μg of protein/ml. The ligand binding specificities of CD36 and SR-BI, determined by direct binding or competition assays, were similar, but not identical; both bind modified proteins (acetylated LDL, oxidized LDL, maleylated bovine serum albumin), but not the broad array of other polyanions (e.g. fucoidin, polyguanosinic acid, carrageenan) which are ligands of the class A receptors. Thus, SR-BI and CD36 define a second class of scavenger receptors, designated class B. Native LDL, which does not bind to either class A receptors or CD36, unexpectedly bound with high affinity to SR-BI. Northern blot analysis of murine tissues showed that SR-BI was most abundantly expressed in fat and was present at moderate levels in lung and liver. Furthermore, SR-BI mRNA expression was induced upon differentiation of 3T3-L1 cells into adipocytes. Thus, the tissue distribution of expression and ligand binding properties of SR-BI raise the possibility that this cell surface receptor may play an important role in lipid metabolism.

Original languageEnglish (US)
Pages (from-to)21003-21009
Number of pages7
JournalJournal of Biological Chemistry
Volume269
Issue number33
StatePublished - Aug 19 1994

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Class B Scavenger Receptors
Cloning
Organism Cloning
Scavenger Receptors
Membrane Proteins
Ligands
Class A Scavenger Receptors
Tissue
3T3-L1 Cells
Proteins
Carrageenan
Cell Surface Receptors
Tissue Distribution
Endocytosis
Cricetulus
Lipid Metabolism
Adipocytes
Northern Blotting
Liver
Lipoproteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Expression cloning of SR-BI, a CD36-related class B scavenger receptor. / Acton, Susan L.; Scherer, Philipp E.; Lodish, Harvey F.; Krieger, Monty.

In: Journal of Biological Chemistry, Vol. 269, No. 33, 19.08.1994, p. 21003-21009.

Research output: Contribution to journalArticle

Acton, SL, Scherer, PE, Lodish, HF & Krieger, M 1994, 'Expression cloning of SR-BI, a CD36-related class B scavenger receptor', Journal of Biological Chemistry, vol. 269, no. 33, pp. 21003-21009.
Acton, Susan L. ; Scherer, Philipp E. ; Lodish, Harvey F. ; Krieger, Monty. / Expression cloning of SR-BI, a CD36-related class B scavenger receptor. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 33. pp. 21003-21009.
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