Expression in lung cancer of a transcribed sequence at the DNF15S2 locus at chromosome 3P21

J. W. Harbour, S. L. Lai, A. F. Gazdar, J. D. Minna, F. J. Kaye

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4 Scopus citations

Abstract

The short arm of chromosome 3 undergoes genetic loss in virtually all small cell lung cancers (SCLC). The most frequently deleted region includes the DNF15S2 locus (mapped to 3p21), suggesting that a putative recessive SCLC gene might be located nearby. Using a DNA probe subcloned from the DNF15S2 locus, were identified a 3.0 kb mRNA species and isolated corresponding partial cDNA clones. Analysis of the nucleic acid sequence showed it to be the same as a recently published transcribed gene isolated from this chromosomal 3p21 region. To determine whether this gene might represent a 'recessive oncogene' we examined a large number of SCLC and other human tumors and found no evidence for DNA rearrangements or inactivation of RNA expression. We discuss the significance of this transcribed gene and the DNF15S2 locus in tumorigenesis.

Original languageEnglish (US)
Pages (from-to)23-27
Number of pages5
JournalAnticancer Research
Volume10
Issue number1
Publication statusPublished - 1990

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Keywords

  • chromosome 3
  • restriction fragment length polymorphism
  • small cell carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Harbour, J. W., Lai, S. L., Gazdar, A. F., Minna, J. D., & Kaye, F. J. (1990). Expression in lung cancer of a transcribed sequence at the DNF15S2 locus at chromosome 3P21. Anticancer Research, 10(1), 23-27.