Expression mosaic of odorant-binding proteins in Drosophila olfactory organs

S. R. Shanbhag; D. Hekmat-Scafe; M. S. Kim; S. K. Park; J. R. Carlson; C. Pikielny; D. P. Smith; R. A. Steinbrecht

doi:10.1002/jemt.1179

Expression mosaic of odorant-binding proteins in Drosophila olfactory organs

S. R. Shanbhag, D. Hekmat-Scafe, M. S. Kim, S. K. Park, J. R. Carlson, C. Pikielny, D. P. Smith, R. A. Steinbrecht

Research output: Contribution to journal › Article › peer-review

101 Scopus citations

Abstract

Deciphering the genome of the fruitfly, Drosophila melanogaster, has revealed 39 genes coding for putative odorant-binding proteins (OBPs), more than are known at present for any other insect species. Using specific antibodies, the expression mosaic of five such OBPs (OS-E, OS-F, LUSH, PBPRP2, PBPRP5) on the antenna and maxillary palp has been mapped in the electron microscope. It was found that (1) OBP expression does correlate with morphological sensillum types and subtypes, (2) several OBPs may be co-localized in the same sensillum, and (3) OBP localization is not restricted to olfactory sensilla. The expression of PBPRP2 in antennal epidermis sheds some light on the possible evolution of OBPs.

Original language	English (US)
Pages (from-to)	297-306
Number of pages	10
Journal	Microscopy Research and Technique
Volume	55
Issue number	5
DOIs	https://doi.org/10.1002/jemt.1179
State	Published - Dec 1 2001

Keywords

Antenna
Immunoelectron microscopy
Maxillary palp
Olfaction
Sensillum basiconicum
Sensillum coeloconicum
Sensillum trichodeum

ASJC Scopus subject areas

Anatomy
Histology
Instrumentation
Medical Laboratory Technology

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10.1002/jemt.1179

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title = "Expression mosaic of odorant-binding proteins in Drosophila olfactory organs",

abstract = "Deciphering the genome of the fruitfly, Drosophila melanogaster, has revealed 39 genes coding for putative odorant-binding proteins (OBPs), more than are known at present for any other insect species. Using specific antibodies, the expression mosaic of five such OBPs (OS-E, OS-F, LUSH, PBPRP2, PBPRP5) on the antenna and maxillary palp has been mapped in the electron microscope. It was found that (1) OBP expression does correlate with morphological sensillum types and subtypes, (2) several OBPs may be co-localized in the same sensillum, and (3) OBP localization is not restricted to olfactory sensilla. The expression of PBPRP2 in antennal epidermis sheds some light on the possible evolution of OBPs.",

keywords = "Antenna, Immunoelectron microscopy, Maxillary palp, Olfaction, Sensillum basiconicum, Sensillum coeloconicum, Sensillum trichodeum",

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AU - Shanbhag, S. R.

AU - Hekmat-Scafe, D.

AU - Kim, M. S.

AU - Park, S. K.

AU - Carlson, J. R.

AU - Pikielny, C.

AU - Smith, D. P.

AU - Steinbrecht, R. A.

PY - 2001/12/1

Y1 - 2001/12/1

N2 - Deciphering the genome of the fruitfly, Drosophila melanogaster, has revealed 39 genes coding for putative odorant-binding proteins (OBPs), more than are known at present for any other insect species. Using specific antibodies, the expression mosaic of five such OBPs (OS-E, OS-F, LUSH, PBPRP2, PBPRP5) on the antenna and maxillary palp has been mapped in the electron microscope. It was found that (1) OBP expression does correlate with morphological sensillum types and subtypes, (2) several OBPs may be co-localized in the same sensillum, and (3) OBP localization is not restricted to olfactory sensilla. The expression of PBPRP2 in antennal epidermis sheds some light on the possible evolution of OBPs.

AB - Deciphering the genome of the fruitfly, Drosophila melanogaster, has revealed 39 genes coding for putative odorant-binding proteins (OBPs), more than are known at present for any other insect species. Using specific antibodies, the expression mosaic of five such OBPs (OS-E, OS-F, LUSH, PBPRP2, PBPRP5) on the antenna and maxillary palp has been mapped in the electron microscope. It was found that (1) OBP expression does correlate with morphological sensillum types and subtypes, (2) several OBPs may be co-localized in the same sensillum, and (3) OBP localization is not restricted to olfactory sensilla. The expression of PBPRP2 in antennal epidermis sheds some light on the possible evolution of OBPs.

KW - Antenna

KW - Immunoelectron microscopy

KW - Maxillary palp

KW - Olfaction

KW - Sensillum basiconicum

KW - Sensillum coeloconicum

KW - Sensillum trichodeum

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ER -

Expression mosaic of odorant-binding proteins in Drosophila olfactory organs

Abstract

Keywords

ASJC Scopus subject areas

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