Expression of β-1,4-galactosyltransferase-I affects cellular adhesion in human peripheral blood CD4+ T cells

Yu Han, Xiaorong Zhou, Yuhong Ji, Aiguo Shen, Xiaolei Sun, Yingqing Hu, Qiong Wu, Xiaoying Wang

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

β-1,4-galactosyltransferase-I (β-1,4-GalT-I) has two isoforms that differ only in the length of their cytoplasmic domains. In this study, we found that both the long and short isoforms of β-1,4-GalT-I were expressed in human CD4+ T lymphocytes, and localized in the cytoplasm and on the plasma membrane. The expression level of β-1,4-GalT-I was increased in CD4+ T cells after stimulation with interleukin (IL)-2, and was further increased after stimulation with IL-2 + IL-12, but decreased after stimulation with IL-2 + IL-4 when compared to stimulation with IL-2 alone. We also demonstrated that the cellular adhesion of CD4+ T cells was significantly increased upon cytokine stimulation, and was inhibited by α-lactalbumin, indicating that the increase in adhesion was positively correlated with the expression and activity of long β-1,4-GalT-I. Collectively, the data suggest that β-1,4-GalT-I plays a role in the cellular adhesion of CD4+ T cells.

Original languageEnglish (US)
Pages (from-to)11-17
Number of pages7
JournalCellular Immunology
Volume262
Issue number1
DOIs
StatePublished - Mar 2 2010

Keywords

  • CD4 helper T lymphocytes
  • Cellular adhesion
  • β-1,4-galactosyltransferase-I

ASJC Scopus subject areas

  • Immunology

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