Expression of β-1,4-galactosyltransferase-I affects cellular adhesion in human peripheral blood CD4+ T cells

Yu Han, Xiaorong Zhou, Yuhong Ji, Aiguo Shen, Xiaolei Sun, Yingqing Hu, Qiong Wu, Xiaoying Wang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

β-1,4-galactosyltransferase-I (β-1,4-GalT-I) has two isoforms that differ only in the length of their cytoplasmic domains. In this study, we found that both the long and short isoforms of β-1,4-GalT-I were expressed in human CD4+ T lymphocytes, and localized in the cytoplasm and on the plasma membrane. The expression level of β-1,4-GalT-I was increased in CD4+ T cells after stimulation with interleukin (IL)-2, and was further increased after stimulation with IL-2 + IL-12, but decreased after stimulation with IL-2 + IL-4 when compared to stimulation with IL-2 alone. We also demonstrated that the cellular adhesion of CD4+ T cells was significantly increased upon cytokine stimulation, and was inhibited by α-lactalbumin, indicating that the increase in adhesion was positively correlated with the expression and activity of long β-1,4-GalT-I. Collectively, the data suggest that β-1,4-GalT-I plays a role in the cellular adhesion of CD4+ T cells.

Original languageEnglish (US)
Pages (from-to)11-17
Number of pages7
JournalCellular Immunology
Volume262
Issue number1
DOIs
StatePublished - 2010

Fingerprint

Interleukin-2
T-Lymphocytes
Protein Isoforms
Lactalbumin
Interleukin-12
Interleukin-4
Cytoplasm
Cell Membrane
triaosylceramide 1,4-galactosyltransferase
xylosylprotein 4-beta-galactosyltransferase
Cytokines

Keywords

  • β-1,4-galactosyltransferase-I
  • CD4 helper T lymphocytes
  • Cellular adhesion

ASJC Scopus subject areas

  • Immunology

Cite this

Expression of β-1,4-galactosyltransferase-I affects cellular adhesion in human peripheral blood CD4+ T cells. / Han, Yu; Zhou, Xiaorong; Ji, Yuhong; Shen, Aiguo; Sun, Xiaolei; Hu, Yingqing; Wu, Qiong; Wang, Xiaoying.

In: Cellular Immunology, Vol. 262, No. 1, 2010, p. 11-17.

Research output: Contribution to journalArticle

Han, Yu ; Zhou, Xiaorong ; Ji, Yuhong ; Shen, Aiguo ; Sun, Xiaolei ; Hu, Yingqing ; Wu, Qiong ; Wang, Xiaoying. / Expression of β-1,4-galactosyltransferase-I affects cellular adhesion in human peripheral blood CD4+ T cells. In: Cellular Immunology. 2010 ; Vol. 262, No. 1. pp. 11-17.
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AU - Zhou, Xiaorong

AU - Ji, Yuhong

AU - Shen, Aiguo

AU - Sun, Xiaolei

AU - Hu, Yingqing

AU - Wu, Qiong

AU - Wang, Xiaoying

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AB - β-1,4-galactosyltransferase-I (β-1,4-GalT-I) has two isoforms that differ only in the length of their cytoplasmic domains. In this study, we found that both the long and short isoforms of β-1,4-GalT-I were expressed in human CD4+ T lymphocytes, and localized in the cytoplasm and on the plasma membrane. The expression level of β-1,4-GalT-I was increased in CD4+ T cells after stimulation with interleukin (IL)-2, and was further increased after stimulation with IL-2 + IL-12, but decreased after stimulation with IL-2 + IL-4 when compared to stimulation with IL-2 alone. We also demonstrated that the cellular adhesion of CD4+ T cells was significantly increased upon cytokine stimulation, and was inhibited by α-lactalbumin, indicating that the increase in adhesion was positively correlated with the expression and activity of long β-1,4-GalT-I. Collectively, the data suggest that β-1,4-GalT-I plays a role in the cellular adhesion of CD4+ T cells.

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