Expression of β-1,4-galactosyltransferase I in CD4+ T H cells and its effect on cellular adhesion ability

Yu Han, Xiao Ying Wang, Yu Hong Ji, Ai Guo Shen, Xiao Lei Sun, Ying Qing Hu, Xiao Rang Zhou, Qiong Wu

Research output: Contribution to journalArticle

Abstract

Objective: To study the expression and localization of β-1,4- galactosyltransferase I (β-1,4-GalT I) in CD4+ helper T(T H)lymphocytes and the effect of β-1,4-GalT I on adhesion ability of CD4+ TH cells. Methods: CD4+ TH cells were isolated by CD4 positive immunomagnetic beads. The expression and localization of β-1,4-GalT I in CD4+ TH cells was detected by RT-PCR, immunofluorescence and laser scanning confocal microscopy. Activation and differentiation of CD4+ TH cells were introduced by cytokines such as rhIL-2, rhIL-12 and rhIL-4. The expression level of β-1,4-GalT I in CD4+ TH cells was measured by semi-quantitative RT-PCR and flow cytometry. The adhesion ability of CD4 + TH cells was compared by cell adhesion assay. α-lactalbumin was used to interfere the activity and substrate specificity of cell surface β-1,4-GalT I, then the influence of that on the adhesion ability of CD4+ TH cells was analyzed by cell adhesion assay. Results: Long and short β-1,4-GalT I were expressed and localized on the cytomembrane and cytoplasm of CD4+ TH cells. The expression level of long β-1,4-GalT I and cell surface β-1,4-GalT I, and the adhesion ability of CD4+ TH cells activated with rhIL-2 increased as compared with unactivated CD4+ TH cells. The expression level of long β-1,4-GalT I and cell surface β-1,4-GalT I were both positively correlated with the adhesion ability of CD4+ TH cells. The adhesion ability of CD4+ TH cells decreased after the incubation with α-lactalbumin. Conclusion: β-1,4-GalT I expression in CD4+ TH cells is closely correlated with cell adhesion ability, which suggests that β-1,4-GalT I may play an important role in the adhesion process of CD4 + TH cells.

Original languageEnglish (US)
Pages (from-to)871-877
Number of pages7
JournalChinese Journal of Microbiology and Immunology
Volume27
Issue number10
StatePublished - Oct 31 2007

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Cell Adhesion
Lactalbumin
xylosylprotein 4-beta-galactosyltransferase
triaosylceramide 1,4-galactosyltransferase
Polymerase Chain Reaction
Substrate Specificity
Confocal Microscopy
Fluorescent Antibody Technique
Flow Cytometry
Cytoplasm
Lymphocytes
Cytokines

Keywords

  • β-1,4-galactosyltransferase I
  • CD4 helper T lymphocytes
  • Cellular adhesion

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)

Cite this

Han, Y., Wang, X. Y., Ji, Y. H., Shen, A. G., Sun, X. L., Hu, Y. Q., ... Wu, Q. (2007). Expression of β-1,4-galactosyltransferase I in CD4+ T H cells and its effect on cellular adhesion ability. Chinese Journal of Microbiology and Immunology, 27(10), 871-877.

Expression of β-1,4-galactosyltransferase I in CD4+ T H cells and its effect on cellular adhesion ability. / Han, Yu; Wang, Xiao Ying; Ji, Yu Hong; Shen, Ai Guo; Sun, Xiao Lei; Hu, Ying Qing; Zhou, Xiao Rang; Wu, Qiong.

In: Chinese Journal of Microbiology and Immunology, Vol. 27, No. 10, 31.10.2007, p. 871-877.

Research output: Contribution to journalArticle

Han, Y, Wang, XY, Ji, YH, Shen, AG, Sun, XL, Hu, YQ, Zhou, XR & Wu, Q 2007, 'Expression of β-1,4-galactosyltransferase I in CD4+ T H cells and its effect on cellular adhesion ability', Chinese Journal of Microbiology and Immunology, vol. 27, no. 10, pp. 871-877.
Han, Yu ; Wang, Xiao Ying ; Ji, Yu Hong ; Shen, Ai Guo ; Sun, Xiao Lei ; Hu, Ying Qing ; Zhou, Xiao Rang ; Wu, Qiong. / Expression of β-1,4-galactosyltransferase I in CD4+ T H cells and its effect on cellular adhesion ability. In: Chinese Journal of Microbiology and Immunology. 2007 ; Vol. 27, No. 10. pp. 871-877.
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abstract = "Objective: To study the expression and localization of β-1,4- galactosyltransferase I (β-1,4-GalT I) in CD4+ helper T(T H)lymphocytes and the effect of β-1,4-GalT I on adhesion ability of CD4+ TH cells. Methods: CD4+ TH cells were isolated by CD4 positive immunomagnetic beads. The expression and localization of β-1,4-GalT I in CD4+ TH cells was detected by RT-PCR, immunofluorescence and laser scanning confocal microscopy. Activation and differentiation of CD4+ TH cells were introduced by cytokines such as rhIL-2, rhIL-12 and rhIL-4. The expression level of β-1,4-GalT I in CD4+ TH cells was measured by semi-quantitative RT-PCR and flow cytometry. The adhesion ability of CD4 + TH cells was compared by cell adhesion assay. α-lactalbumin was used to interfere the activity and substrate specificity of cell surface β-1,4-GalT I, then the influence of that on the adhesion ability of CD4+ TH cells was analyzed by cell adhesion assay. Results: Long and short β-1,4-GalT I were expressed and localized on the cytomembrane and cytoplasm of CD4+ TH cells. The expression level of long β-1,4-GalT I and cell surface β-1,4-GalT I, and the adhesion ability of CD4+ TH cells activated with rhIL-2 increased as compared with unactivated CD4+ TH cells. The expression level of long β-1,4-GalT I and cell surface β-1,4-GalT I were both positively correlated with the adhesion ability of CD4+ TH cells. The adhesion ability of CD4+ TH cells decreased after the incubation with α-lactalbumin. Conclusion: β-1,4-GalT I expression in CD4+ TH cells is closely correlated with cell adhesion ability, which suggests that β-1,4-GalT I may play an important role in the adhesion process of CD4 + TH cells.",
keywords = "β-1,4-galactosyltransferase I, CD4 helper T lymphocytes, Cellular adhesion",
author = "Yu Han and Wang, {Xiao Ying} and Ji, {Yu Hong} and Shen, {Ai Guo} and Sun, {Xiao Lei} and Hu, {Ying Qing} and Zhou, {Xiao Rang} and Qiong Wu",
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T1 - Expression of β-1,4-galactosyltransferase I in CD4+ T H cells and its effect on cellular adhesion ability

AU - Han, Yu

AU - Wang, Xiao Ying

AU - Ji, Yu Hong

AU - Shen, Ai Guo

AU - Sun, Xiao Lei

AU - Hu, Ying Qing

AU - Zhou, Xiao Rang

AU - Wu, Qiong

PY - 2007/10/31

Y1 - 2007/10/31

N2 - Objective: To study the expression and localization of β-1,4- galactosyltransferase I (β-1,4-GalT I) in CD4+ helper T(T H)lymphocytes and the effect of β-1,4-GalT I on adhesion ability of CD4+ TH cells. Methods: CD4+ TH cells were isolated by CD4 positive immunomagnetic beads. The expression and localization of β-1,4-GalT I in CD4+ TH cells was detected by RT-PCR, immunofluorescence and laser scanning confocal microscopy. Activation and differentiation of CD4+ TH cells were introduced by cytokines such as rhIL-2, rhIL-12 and rhIL-4. The expression level of β-1,4-GalT I in CD4+ TH cells was measured by semi-quantitative RT-PCR and flow cytometry. The adhesion ability of CD4 + TH cells was compared by cell adhesion assay. α-lactalbumin was used to interfere the activity and substrate specificity of cell surface β-1,4-GalT I, then the influence of that on the adhesion ability of CD4+ TH cells was analyzed by cell adhesion assay. Results: Long and short β-1,4-GalT I were expressed and localized on the cytomembrane and cytoplasm of CD4+ TH cells. The expression level of long β-1,4-GalT I and cell surface β-1,4-GalT I, and the adhesion ability of CD4+ TH cells activated with rhIL-2 increased as compared with unactivated CD4+ TH cells. The expression level of long β-1,4-GalT I and cell surface β-1,4-GalT I were both positively correlated with the adhesion ability of CD4+ TH cells. The adhesion ability of CD4+ TH cells decreased after the incubation with α-lactalbumin. Conclusion: β-1,4-GalT I expression in CD4+ TH cells is closely correlated with cell adhesion ability, which suggests that β-1,4-GalT I may play an important role in the adhesion process of CD4 + TH cells.

AB - Objective: To study the expression and localization of β-1,4- galactosyltransferase I (β-1,4-GalT I) in CD4+ helper T(T H)lymphocytes and the effect of β-1,4-GalT I on adhesion ability of CD4+ TH cells. Methods: CD4+ TH cells were isolated by CD4 positive immunomagnetic beads. The expression and localization of β-1,4-GalT I in CD4+ TH cells was detected by RT-PCR, immunofluorescence and laser scanning confocal microscopy. Activation and differentiation of CD4+ TH cells were introduced by cytokines such as rhIL-2, rhIL-12 and rhIL-4. The expression level of β-1,4-GalT I in CD4+ TH cells was measured by semi-quantitative RT-PCR and flow cytometry. The adhesion ability of CD4 + TH cells was compared by cell adhesion assay. α-lactalbumin was used to interfere the activity and substrate specificity of cell surface β-1,4-GalT I, then the influence of that on the adhesion ability of CD4+ TH cells was analyzed by cell adhesion assay. Results: Long and short β-1,4-GalT I were expressed and localized on the cytomembrane and cytoplasm of CD4+ TH cells. The expression level of long β-1,4-GalT I and cell surface β-1,4-GalT I, and the adhesion ability of CD4+ TH cells activated with rhIL-2 increased as compared with unactivated CD4+ TH cells. The expression level of long β-1,4-GalT I and cell surface β-1,4-GalT I were both positively correlated with the adhesion ability of CD4+ TH cells. The adhesion ability of CD4+ TH cells decreased after the incubation with α-lactalbumin. Conclusion: β-1,4-GalT I expression in CD4+ TH cells is closely correlated with cell adhesion ability, which suggests that β-1,4-GalT I may play an important role in the adhesion process of CD4 + TH cells.

KW - β-1,4-galactosyltransferase I

KW - CD4 helper T lymphocytes

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