Expression of a class 1 knotted1-like homeobox gene is down-regulated in pea compound leaf primordia

Julie Hofer, Campbell Gourlay, Anthony Michael, T. H Noel Ellis

Research output: Contribution to journalArticle

84 Scopus citations

Abstract

Differences in knotted1-like (knox) gene expression may account for some of the diversity of leaf forms seen in nature. Class 1 knox genes are expressed in the compound leaf primordia of tomato but not in the simple leaf primordia of a range of species examined so far. In order to test the hypothesis that all compound leaves differ from simple leaves in this way, we isolated a class 1 knox cDNA from pea, Pskn1 (Pisum sativum knotted1) and examined its expression pattern. The encoded homeodomain of Pskn1 shares 88% identical residues with KNOTTED1 from maize and an adjacent ELK domain is present. The protein sequence of PSKN1 is 69% identical to TKN2, its nearest related sequence in tomato. Unlike TKn2, Pskn1 was not expressed in newly initiated compound leaves. The expression pattern of Pskn1 resembled those of other class 1 knox genes described in maize and Arabidopsis. Transcripts were detected in the shoot apical meristem and developing vasculature of the vegetative shoot, but expression was not detected in newly initiated and developing compound leaf primordia. The same pattern of expression was observed in the afila mutant, which is characterised by highly ramified compound leaves. Our results suggest that tomato and pea use different developmental processes in the generation of their compound leaves.

Original languageEnglish (US)
Article number310357
Pages (from-to)387-398
Number of pages12
JournalPlant Molecular Biology
Volume45
Issue number4
DOIs
StatePublished - Jan 1 2001

Keywords

  • Compound leaf
  • Homeobox
  • Pea
  • Plant development

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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