Expression of a microinjected immunoglobulin gene in the spleen of transgenic mice

Ralph L. Brinster, Kindred A. Ritchie, Robert E Hammer, Rebecca L. O'Brien, Benjamin Arp, Ursula Storb

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Transgenic mice were produced by microinjection of a rearranged, functional immunoglobulin κ gene into fertilized mouse eggs and implantation of the microinjected embryos into foster mothers. Mice that integrated the injected gene were mated and the DNA, RNA and serum κ chains of their offspring were analysed. The data from offspring of three different transgenic mice indicate that the microinjected gene is expressed in the spleen, but not the liver of mice which inherited the injected gene.

Original languageEnglish (US)
Pages (from-to)332-336
Number of pages5
JournalNature
Volume306
Issue number5941
DOIs
StatePublished - 1983

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Immunoglobulin Genes
Transgenic Mice
Spleen
Genes
Zygote
Microinjections
RNA
Liver
DNA
Serum

ASJC Scopus subject areas

  • General

Cite this

Brinster, R. L., Ritchie, K. A., Hammer, R. E., O'Brien, R. L., Arp, B., & Storb, U. (1983). Expression of a microinjected immunoglobulin gene in the spleen of transgenic mice. Nature, 306(5941), 332-336. https://doi.org/10.1038/306332a0

Expression of a microinjected immunoglobulin gene in the spleen of transgenic mice. / Brinster, Ralph L.; Ritchie, Kindred A.; Hammer, Robert E; O'Brien, Rebecca L.; Arp, Benjamin; Storb, Ursula.

In: Nature, Vol. 306, No. 5941, 1983, p. 332-336.

Research output: Contribution to journalArticle

Brinster, RL, Ritchie, KA, Hammer, RE, O'Brien, RL, Arp, B & Storb, U 1983, 'Expression of a microinjected immunoglobulin gene in the spleen of transgenic mice', Nature, vol. 306, no. 5941, pp. 332-336. https://doi.org/10.1038/306332a0
Brinster, Ralph L. ; Ritchie, Kindred A. ; Hammer, Robert E ; O'Brien, Rebecca L. ; Arp, Benjamin ; Storb, Ursula. / Expression of a microinjected immunoglobulin gene in the spleen of transgenic mice. In: Nature. 1983 ; Vol. 306, No. 5941. pp. 332-336.
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