Expression of BCR/ABL p210 from a Knockin Allele Enhances Bone Marrow Engraftment without Inducing Neoplasia

Samantha B. Foley, Zacariah L. Hildenbrand, Abigail A. Soyombo, Jeffery A. Magee, Yipin Wu, Katherine I. Oravecz-Wilson, Theodora S. Ross

Research output: Contribution to journalArticle

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Abstract

Chronic myeloid leukemia (CML) and some acute lymphoblastic leukemias are characterized by the t(9;22) chromosome, which encodes the BCR/ABL oncogene. Multiple mouse models of CML express BCR/ABL at high levels from non-. Bcr promoters, resulting in the development of leukemias. In contrast, a significant fraction of healthy humans have been found to have BCR/ABL-positive hematopoietic cells. To bridge the gap between the information derived from current mouse models and nonleukemic humans with the BCR/ABL oncogene, we generated a knockin model with BCR/ABL p210 expressed from the Bcr locus. Unlike previous models, expression of BCR/ABL from the knockin allele did not induce leukemia. BCR/ABL mutant cells did exhibit favorable bone marrow engraftment compared to control cells. These data suggest that BCR/ABL expression alone is insufficient to induce disease. This model allows for inducible spatial and temporal control of BCR/ABL expression for analysis of early steps in the pathogenesis of BCR/ABL-expressing leukemias

Original languageEnglish (US)
Pages (from-to)51-60
Number of pages10
JournalCell Reports
Volume5
Issue number1
DOIs
StatePublished - Oct 17 2013

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Bone
Leukemia
Bone Marrow
Alleles
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Oncogenes
Chromosomes, Human, Pair 22
Neoplasms
Chromosomes, Human, Pair 9
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Chromosomes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Expression of BCR/ABL p210 from a Knockin Allele Enhances Bone Marrow Engraftment without Inducing Neoplasia. / Foley, Samantha B.; Hildenbrand, Zacariah L.; Soyombo, Abigail A.; Magee, Jeffery A.; Wu, Yipin; Oravecz-Wilson, Katherine I.; Ross, Theodora S.

In: Cell Reports, Vol. 5, No. 1, 17.10.2013, p. 51-60.

Research output: Contribution to journalArticle

Foley, Samantha B. ; Hildenbrand, Zacariah L. ; Soyombo, Abigail A. ; Magee, Jeffery A. ; Wu, Yipin ; Oravecz-Wilson, Katherine I. ; Ross, Theodora S. / Expression of BCR/ABL p210 from a Knockin Allele Enhances Bone Marrow Engraftment without Inducing Neoplasia. In: Cell Reports. 2013 ; Vol. 5, No. 1. pp. 51-60.
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