Expression of Borrelia burgdorferi OspC and DbpA is controlled by a RpoN-RpoS regulatory pathway

Anette Hübner, Xiaofeng Yang, Dena M. Nolen, Taissia G. Popova, Felipe C. Cabello, Michael V. Norgard

Research output: Contribution to journalArticle

240 Citations (Scopus)

Abstract

RpoS and RpoN are two alternative sigma factors typically associated with general stress responses in bacteria. To date, there has been no experimental evidence that RpoS and RpoN can directly control the expression of one another. Herein, using a combined strategy of gene disruption and genetic complementation targeting rpoN and rpoS in Borrelia burgdorferi strain 297, we describe a regulatory network for B. burgdorferi. In this network, RpoN controls the expression of RpoS, which, in turn, governs the expression of two important membrane lipoproteins, outer surface protein C and decorin-binding protein A, and likely other proteins of B. burgdorferi. Our findings provide a foundation for elucidating further key regulatory networks that potentially impact many aspects of B. burgdorferi's parasitic strategy, host range, and virulence expression.

Original languageEnglish (US)
Pages (from-to)12724-12729
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number22
DOIs
StatePublished - Oct 23 2001

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Borrelia burgdorferi
Decorin
Sigma Factor
Host Specificity
Staphylococcal Protein A
Lipoproteins
Virulence
Carrier Proteins
Bacteria
Membranes
Genes
IgA receptor
OspC protein

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Expression of Borrelia burgdorferi OspC and DbpA is controlled by a RpoN-RpoS regulatory pathway. / Hübner, Anette; Yang, Xiaofeng; Nolen, Dena M.; Popova, Taissia G.; Cabello, Felipe C.; Norgard, Michael V.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 22, 23.10.2001, p. 12724-12729.

Research output: Contribution to journalArticle

Hübner, Anette ; Yang, Xiaofeng ; Nolen, Dena M. ; Popova, Taissia G. ; Cabello, Felipe C. ; Norgard, Michael V. / Expression of Borrelia burgdorferi OspC and DbpA is controlled by a RpoN-RpoS regulatory pathway. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 22. pp. 12724-12729.
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