Expression of c-myc in progenitor cells of the bronchopulmonary epithelium and in a large number of non-small cell lung cancers.

J. L. Broers, J. Viallet, S. M. Jensen, H. Pass, W. D. Travis, J. D. Minna, R. I. Linnoila

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Abstract

We performed in situ hybridization for c-myc, N-myc, and L-myc mRNA expression using 35S-labeled cRNA probes on frozen sections of 19 pairs of non-small cell lung cancers (NSCLC) and the surrounding non-neoplastic lung tissue. In non-neoplastic lung, c-myc expression was strongest in bronchial epithelium basal cells and hyperplastic alveolar type II pneumocytes, which are potential progenitor cells for bronchopulmonary epithelium and their tumors. In contrast, N-myc and L-myc mRNAs were not detected in non-neoplastic lung. In studies of freshly resected primary tumors, expression of c-myc was detected in 11 of 19 NSCLC (with the highest levels in squamous cell carcinomas), two of which also expressed L-myc, while N-myc expression was never detected. Levels of c-myc expression in tumors were significantly higher than in non-neoplastic lung samples. We conclude that: (1) c-myc expression in non-neoplastic lung tissues is highest in bronchial basal cells and hyperplastic type II cells, and (2) in NSCLC, overexpression of the myc-proto-oncogene is common.

Original languageEnglish (US)
Pages (from-to)33-43
Number of pages11
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume9
Issue number1
StatePublished - Jul 1993

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Non-Small Cell Lung Carcinoma
Tumors
Stem Cells
Epithelium
Cells
Lung
Alveolar Epithelial Cells
Tissue
Complementary RNA
Messenger RNA
Neoplasms
myc Genes
Proto-Oncogenes
Frozen Sections
In Situ Hybridization
Squamous Cell Carcinoma
Epithelial Cells

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Expression of c-myc in progenitor cells of the bronchopulmonary epithelium and in a large number of non-small cell lung cancers. / Broers, J. L.; Viallet, J.; Jensen, S. M.; Pass, H.; Travis, W. D.; Minna, J. D.; Linnoila, R. I.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 9, No. 1, 07.1993, p. 33-43.

Research output: Contribution to journalArticle

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