Expression of carbonic anhydrase IV in carbonic anhydrase II-deficient mice

Luc P. Brion, Wendy Cammer, Lisa M. Satlin, Carlos Suarez, Beth J. Zavilowitz, Victor L. Schuster

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Chronic metabolic acidosis (CMA) in the rabbit upregulates carbonic anhydrase (CA) IV in the proximal convoluted tubule (PCT). This study was designed to assess CA IV expression in a model of CMA in the mouse, i.e., congenital deficiency in CA II [CA(II)D]. In female CA(II)D mice, CA IV specific activity but not CA IV immunoreactivity was upregulated in the renal cortex, specifically in microdissected PCTs. Western blot analysis showed higher expression of CA IV immunoreactive protein in renal membranes from males than in those from females.

Original languageEnglish (US)
Pages (from-to)F234-F245
JournalAmerican Journal of Physiology - Renal Physiology
Volume273
Issue number2 42-2
DOIs
StatePublished - Aug 1997

Keywords

  • Affinity chromatography
  • Carbonic anhydrase II deficiency
  • Carbonic anhydrase isozymes I, II, III, and IV
  • Cortex
  • Hydratase activity
  • Liver
  • Lung
  • Outer medulla
  • Regulation
  • Renal tubular acidosis

ASJC Scopus subject areas

  • Physiology
  • Urology

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