Expression of caveolin-1 and -2 in differentiating PC12 cells and dorsal root ganglion neurons: Caveolin-2 is up-regulated in response to cell injury

F. Galbiati, D. Volonte, O. Gil, G. Zanazzi, J. L. Salzer, M. Sargiacomo, P. E. Scherer, J. A. Engelman, A. Schlegel, M. Parenti, T. Okamoto, M. P. Lisanti

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

Caveolae are cholesterol/sphingolipid-rich microdomains of the plasma membrane that have been implicated in signal transduction and vesicular trafficking. Caveolins are a family of caveolae-associated integral membrane proteins. Caveolin-1 and -2 show the widest range of expression, whereas caveolin-3 expression is restricted to muscle cell types. It has been previously reported that little or no caveolin mRNA species are detectable in the brain by Northern blot analyses or in neuroblastoma cell lines. However, it remains unknown whether caveolins are expressed within neuronal cells. Here we demonstrate the expression of caveolin-1 and -2 in differentiating PC12 cells and dorsal root ganglion (DRG) neurons by using mono-specific antibody probes. In PC12 cells, caveolin-1 expression is up-regulated on day 4 of nerve growth factor (NGF) treatment, whereas caveolin-2 expression is transiently up-regulated early in the differentiation program and then rapidly down-regulated. Interestingly, caveolin-2 is up-regulated in response to the mechanical injury of differentiated PC12 cells; up-regulation of caveolin-2 under these conditions is strictly dependent on continued treatment with NGF. Robust expression of caveolin-1 and -2 is also observed along the entire cell surface of DRG neurons, including high levels on growth cones. These findings demonstrate that neuronal cells express caveolins.

Original languageEnglish (US)
Pages (from-to)10257-10262
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number17
DOIs
StatePublished - Aug 18 1998

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Caveolin 2
Caveolin 1
PC12 Cells
Spinal Ganglia
Caveolins
Neurons
Wounds and Injuries
Caveolae
Nerve Growth Factor
Caveolin 3
Membrane Microdomains
Growth Cones
Neuroblastoma
Northern Blotting
Muscle Cells
Signal Transduction
Membrane Proteins
Up-Regulation
Cholesterol
Cell Membrane

ASJC Scopus subject areas

  • Genetics
  • General

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Expression of caveolin-1 and -2 in differentiating PC12 cells and dorsal root ganglion neurons : Caveolin-2 is up-regulated in response to cell injury. / Galbiati, F.; Volonte, D.; Gil, O.; Zanazzi, G.; Salzer, J. L.; Sargiacomo, M.; Scherer, P. E.; Engelman, J. A.; Schlegel, A.; Parenti, M.; Okamoto, T.; Lisanti, M. P.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 17, 18.08.1998, p. 10257-10262.

Research output: Contribution to journalArticle

Galbiati, F. ; Volonte, D. ; Gil, O. ; Zanazzi, G. ; Salzer, J. L. ; Sargiacomo, M. ; Scherer, P. E. ; Engelman, J. A. ; Schlegel, A. ; Parenti, M. ; Okamoto, T. ; Lisanti, M. P. / Expression of caveolin-1 and -2 in differentiating PC12 cells and dorsal root ganglion neurons : Caveolin-2 is up-regulated in response to cell injury. In: Proceedings of the National Academy of Sciences of the United States of America. 1998 ; Vol. 95, No. 17. pp. 10257-10262.
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AU - Gil, O.

AU - Zanazzi, G.

AU - Salzer, J. L.

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