Expression of Connective Tissue Growth Factor after Glaucoma Filtration Surgery in a Rabbit Model

Douglas W. Esson, Arvind Neelakantan, Sandhya A. Iyer, Timothy D. Blalock, Lakshmi Balasubramanian, Gary R. Grotendorst, Gregory S. Schultz, Mark B. Sherwood

Research output: Contribution to journalArticle

67 Scopus citations

Abstract

PURPOSE. Connective tissue growth factor (CTGF) appears to play a significant role in mediating fibrosis in several tissues. To gain further understanding of the role of CTGF in the scar formation that occurs after glaucoma filtering surgery (GFS), experiments were performed in a rabbit model. METHODS. Three experiments were performed: (1) CTGF and transforming growth factor (TGF)-β expression were measured quantitatively after GFS, using ELISA. (2) After GFS conjunctival bleb tissues were immunostained for the presence of CTGF and TGF-β. (3) Exogenous CTGF was injected into mitomycin-C (MMC)-treated filtering blebs and the scaring response compared to TGF-β and physiological saline-injected blebs. RESULTS. CTGF and TGF-β were expressed maximally by day 5 after surgery and were both shown to be present in the bleb tissues after GFS. The addition of exogenous CTGF and TGF-β increased the rate of failure of GFS blebs. CONCLUSIONS. These data support the hypothesis that CTGF plays an important role in scarring and wound contracture after GFS. Inhibition of CTGF synthesis or its action may help prevent bleb failure and improve long-term GFS outcomes.

Original languageEnglish (US)
Pages (from-to)485-491
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume45
Issue number2
DOIs
StatePublished - Feb 1 2004

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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    Esson, D. W., Neelakantan, A., Iyer, S. A., Blalock, T. D., Balasubramanian, L., Grotendorst, G. R., Schultz, G. S., & Sherwood, M. B. (2004). Expression of Connective Tissue Growth Factor after Glaucoma Filtration Surgery in a Rabbit Model. Investigative Ophthalmology and Visual Science, 45(2), 485-491. https://doi.org/10.1167/iovs.03-0485