Expression of Functional Ribozymes in Bacteria

Ruth Levitz, Jian Ying Wang, Mouldy Sioud, Karl Drlica

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A trans-acting hammerhead ribozyme, designed to cleave mRNA from the human immunodeficiency virus-1 integrase gene, was expressed in Escherichia coli from a plasmid-borne gene. Expression of the ribozyme at the same time as expression of integrase led to the disappearance of integrase mRNA and prevention of integrase production. A comparable antisense RNA was much less effective. The elements of the ribozyme gene are described to facilitate construction of ribozymes directed at other targets. Procedures for modeling ribozyme secondary structure, testing ribozyme activity, and inserting ribozyme genes into expression vectors are discussed.

Original languageEnglish (US)
Pages (from-to)29-35
Number of pages7
JournalMethods
Volume5
Issue number1
DOIs
StatePublished - Apr 1993

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Catalytic RNA
Integrases
Bacteria
Genes
Antisense RNA
Messenger RNA
Viruses
Gene expression
Escherichia coli
HIV-1
Plasmids
Gene Expression
Testing

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Levitz, R., Wang, J. Y., Sioud, M., & Drlica, K. (1993). Expression of Functional Ribozymes in Bacteria. Methods, 5(1), 29-35. https://doi.org/10.1006/meth.1993.1004

Expression of Functional Ribozymes in Bacteria. / Levitz, Ruth; Wang, Jian Ying; Sioud, Mouldy; Drlica, Karl.

In: Methods, Vol. 5, No. 1, 04.1993, p. 29-35.

Research output: Contribution to journalArticle

Levitz, R, Wang, JY, Sioud, M & Drlica, K 1993, 'Expression of Functional Ribozymes in Bacteria', Methods, vol. 5, no. 1, pp. 29-35. https://doi.org/10.1006/meth.1993.1004
Levitz R, Wang JY, Sioud M, Drlica K. Expression of Functional Ribozymes in Bacteria. Methods. 1993 Apr;5(1):29-35. https://doi.org/10.1006/meth.1993.1004
Levitz, Ruth ; Wang, Jian Ying ; Sioud, Mouldy ; Drlica, Karl. / Expression of Functional Ribozymes in Bacteria. In: Methods. 1993 ; Vol. 5, No. 1. pp. 29-35.
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