Expression of ghrelin receptor mRNA in the rat and the mouse brain

Jeffrey M. Zigman, Juli E. Jones, Charlotte E. Lee, Clifford B. Saper, Joel K. Elmquist

Research output: Contribution to journalArticle

645 Citations (Scopus)

Abstract

Ghrelin is a hormone that stimulates growth hormone secretion and signals energy insufficiency via interaction with its receptor, the growth hormone secretagogue receptor (GHSR). The GHSR is located in both the central nervous system and the periphery. Its distribution in the CNS, as assessed by in situ hybridization histochemistry (ISHH), has been described previously in a few mammalian species, although these studies were limited by either the detail provided or the extent of the regions examined. In the present study, we systematically examined the distribution of GHSR mRNA in the adult rat and mouse brains and cervical spinal cords by using ISHH with novel cRNA probes specific for the mRNA encoding functional GHSR (the type 1a variant). We confirmed GHSR mRNA expression in several hypothalamic nuclei, many of which have long been recognized as playing roles in body weight and food intake. GHSR also was found in several other regions previously unknown to express GHSR mRNA, including many parasympathetic preganglionic neurons. Additionally, we found GHSR mRNA within all three components of the dorsal vagal complex, including the area postrema, the nucleus of the solitary tract, and the dorsal motor nucleus of the vagus. Finally, we examined the coexpression of GHSR with tyrosine hydroxylase and cholecystokinin and demonstrate a high degree of GHSR mRNA expression within dopaminergic, cholecystokinin-containing neurons of the substantia nigra and ventral tegmental area.

Original languageEnglish (US)
Pages (from-to)528-548
Number of pages21
JournalJournal of Comparative Neurology
Volume494
Issue number3
DOIs
StatePublished - Jan 20 2006

Fingerprint

Ghrelin Receptor
Messenger RNA
Brain
Cholecystokinin
In Situ Hybridization
Area Postrema
Neurons
Complementary RNA
Ventral Tegmental Area
Ghrelin
Solitary Nucleus
Tyrosine 3-Monooxygenase
Substantia Nigra
Growth Hormone
Central Nervous System
Eating
Body Weight

Keywords

  • CNS distribution
  • Ghrelin
  • GHSR
  • In situ hybridization histochemistry

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of ghrelin receptor mRNA in the rat and the mouse brain. / Zigman, Jeffrey M.; Jones, Juli E.; Lee, Charlotte E.; Saper, Clifford B.; Elmquist, Joel K.

In: Journal of Comparative Neurology, Vol. 494, No. 3, 20.01.2006, p. 528-548.

Research output: Contribution to journalArticle

Zigman, Jeffrey M. ; Jones, Juli E. ; Lee, Charlotte E. ; Saper, Clifford B. ; Elmquist, Joel K. / Expression of ghrelin receptor mRNA in the rat and the mouse brain. In: Journal of Comparative Neurology. 2006 ; Vol. 494, No. 3. pp. 528-548.
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