Expression of indoleamine 2,3-dioxygenase in metastatic malignant melanoma recruits regulatory T cells to avoid immune detection and affects survival

Jonathan R. Brody, Christina L. Costantino, Adam C. Berger, Takami Sato, Michael P. Lisanti, Charles J. Yeo, Robert V. Emmons, Agnieszka K. Witkiewicz

Research output: Contribution to journalArticle

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Abstract

The mechanism by which malignant melanoma (MM) cells survive in lymph nodes is poorly understood. One possible mechanism by which MM cells can escape immune surveillance is through upregulation of immunomodulatory enzymes such as indoleamine 2,3-dioxygenase (IDO). In this study, 25 cases of MM lymph node metastases from patients with long and short survival were evaluated for expression of IDO and the number of Forkhead box p3 (FOXP3)-expressing regulatory T cells. Moderate to strong cytoplasmic IDO expression was present in all (15/15) MM lymph node metastases in patients with poor survival. Eight of 10 patients with metastatic MM and long survival were negative or only weakly positive for IDO. Upregulation of IDO in metastatic MM cells was associated with an increased number of regulatory T cells (Tregs). There was a statistically significant association between shorter survival and both a stronger IDO expression (p = 0.0019) and a higher number of FOXP3 expressing Tregs (p < 0.001). Using RT-PCR analysis, we showed that IDO expression in MM cells is induced by interferon-gamma. These data support the notion that metastatic MM cells select for expression of IDO to evade immunologic detection. Therefore, inhibition of IDO in MM patients may be a useful treatment strategy.

Original languageEnglish (US)
Pages (from-to)1930-1934
Number of pages5
JournalCell Cycle
Volume8
Issue number12
StatePublished - Jun 15 2009

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Indoleamine-Pyrrole 2,3,-Dioxygenase
Regulatory T-Lymphocytes
Melanoma
Survival
Lymph Nodes
Up-Regulation
Neoplasm Metastasis
Interferon-gamma
Polymerase Chain Reaction

Keywords

  • FOXP3
  • IDO
  • Malignant melanoma
  • Tregs

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Cite this

Expression of indoleamine 2,3-dioxygenase in metastatic malignant melanoma recruits regulatory T cells to avoid immune detection and affects survival. / Brody, Jonathan R.; Costantino, Christina L.; Berger, Adam C.; Sato, Takami; Lisanti, Michael P.; Yeo, Charles J.; Emmons, Robert V.; Witkiewicz, Agnieszka K.

In: Cell Cycle, Vol. 8, No. 12, 15.06.2009, p. 1930-1934.

Research output: Contribution to journalArticle

Brody, JR, Costantino, CL, Berger, AC, Sato, T, Lisanti, MP, Yeo, CJ, Emmons, RV & Witkiewicz, AK 2009, 'Expression of indoleamine 2,3-dioxygenase in metastatic malignant melanoma recruits regulatory T cells to avoid immune detection and affects survival', Cell Cycle, vol. 8, no. 12, pp. 1930-1934.
Brody, Jonathan R. ; Costantino, Christina L. ; Berger, Adam C. ; Sato, Takami ; Lisanti, Michael P. ; Yeo, Charles J. ; Emmons, Robert V. ; Witkiewicz, Agnieszka K. / Expression of indoleamine 2,3-dioxygenase in metastatic malignant melanoma recruits regulatory T cells to avoid immune detection and affects survival. In: Cell Cycle. 2009 ; Vol. 8, No. 12. pp. 1930-1934.
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