Expression of inositol 1,4,5-trisphosphate receptors changes the Ca2+ signal of Xenopus oocytes

Sylvain Delisle, Olivier Blondel, Frank J. Longo, William E. Schnabel, Graeme I. Bell, Michael J. Welsh

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

The receptors for the second messenger inositol 1,4,5-trisphosphate [Ins( 1,4,5)P3] form a family of closely related proteins that play an important role in regulating the free intracellular Ca2+ concentration. To test the hypothesis that changing the expression level of Ins(1,4,5)P3 receptors could alter the Ins(1,4,5)P3-mediated Ca2+ signal, we overexpressed Ins(1,4,5)P3 receptor type 1 (InsP3R-1) or type 3 (InsP3R-3) in Xenopus laevis oocytes. Expression of InsP3R-1 increased the velocity of the propagating waves of intracellular Ca2+ release but did not affect the Ins(1,4,5)P3-induced entry of extracellular Ca2+ across the plasma membrane. In contrast, expression of InsP3R-3 did not affect Ins(1,4,5)P3-induced release of intracellular Ca2+ but markedly increased the magnitude and duration of Ca2+ influx. Immunolocalization studies revealed InsP3R-3 at the endoplasmic reticulum, with a relatively stronger signal at or near the plasma membrane. The results suggest that changing the expression level of an InsP3R can alter the Ins(1,4,5)P3-mediated Ca2+ signal and that InsP3R-1 and InsP3R-3 may have different biological functions.

Original languageEnglish (US)
Pages (from-to)C1255-C1261
JournalAmerican Journal of Physiology - Cell Physiology
Volume270
Issue number4 39-4
StatePublished - Apr 1 1996

Keywords

  • Calcium
  • Calcium entry
  • Calcium signaling
  • Signal transduction

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Fingerprint Dive into the research topics of 'Expression of inositol 1,4,5-trisphosphate receptors changes the Ca<sup>2+</sup> signal of Xenopus oocytes'. Together they form a unique fingerprint.

  • Cite this

    Delisle, S., Blondel, O., Longo, F. J., Schnabel, W. E., Bell, G. I., & Welsh, M. J. (1996). Expression of inositol 1,4,5-trisphosphate receptors changes the Ca2+ signal of Xenopus oocytes. American Journal of Physiology - Cell Physiology, 270(4 39-4), C1255-C1261.