Expression of Le(x) antigen in Schistosoma japonicum and S.haematobium and immune responses to Le(x) in infected animals: Lack of Le(x) expression in other trematodes and nematodes

A. Kwame Nyame, Russell Debose-Boyd, Troy D. Long, Victor C W Tsang, Richard D. Cummings

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Adults of the human parasitic trematode Schistosoma mansoni, which causes hepatosplenic/intestinal complications in humans, synthesize glycoconjugates containing the Lewis x (Le(x)) Galβ1→4(Fucα1→3)GlcNAcβ1→R, but not sialyl Lewis x (sLe(x)), antigen. We now report on our analyses of Le(x) and sLe(x) expression in S.haematobium and S.japonicum, which are two other major species of human schistosomes that cause disease, and the possible autoimmunity to these antigens in infected individuals. Antigen expression was evaluated by both ELISA and Western blot analyses of detergent extracts of parasites using monoclonal antibodies. Several high molecular weight glycoproteins in both S.haematobium and S.japonicum contain the Le(x) antigen, but no sialyl Le(x) antigen was detected. In addition, sera from humans and rodents infected with S.haematobium and S.japonicum contain antibodies reactive with Le(x). These results led us to investigate whether Le(x) antigens are expressed in other helminths, including the parasitic trematode Fasciola hepatica, the parasitic nematode Dirofilaria immitis (dog heartworm), the ruminant nematode Haemonchus contortus, and the free-living nematode Caenorhabditis elegans. Neither Le(x) nor sialyl-Le(x) is detectable in these other helminths. Furthermore, none of the helminths, including schistosomes, express Lea, Leb, Le(y), or the H-type 1 antigen. However, several glycoproteins from all helminths analyzed are bound by Lotus tetragonolobus agglutinin, which binds Fucα1→3GlcNAc, and Wisteria floribunda agglutinin, which binds GalNAcβ1→4GlcNAc (lacdiNAc or LDN). Thus, schistosomes may be unique among helminths in expressing the Le(x) antigen, whereas many different helminths may express α1,3-fucosylated glycans and the LDN motif.

Original languageEnglish (US)
Pages (from-to)615-624
Number of pages10
JournalGlycobiology
Volume8
Issue number6
DOIs
StatePublished - Jun 1998

Fingerprint

Schistosoma japonicum
Helminths
Animals
Antigens
Dirofilaria immitis
Glycoproteins
Haemonchus
Fasciola hepatica
Glycoconjugates
Schistosoma mansoni
Caenorhabditis elegans
Ruminants
Autoimmunity
Detergents
Polysaccharides
Rodentia
Parasites
Molecular Weight
Western Blotting
Enzyme-Linked Immunosorbent Assay

Keywords

  • Caenorhabditis elegans
  • Dirofilaria immitis
  • Fasciola hepatica
  • Haemonchus contortus
  • Lewis x antigen
  • Schistosoma haematobium
  • Schistosoma japonicum
  • Schistosoma mansoni

ASJC Scopus subject areas

  • Biochemistry

Cite this

Expression of Le(x) antigen in Schistosoma japonicum and S.haematobium and immune responses to Le(x) in infected animals : Lack of Le(x) expression in other trematodes and nematodes. / Nyame, A. Kwame; Debose-Boyd, Russell; Long, Troy D.; Tsang, Victor C W; Cummings, Richard D.

In: Glycobiology, Vol. 8, No. 6, 06.1998, p. 615-624.

Research output: Contribution to journalArticle

@article{9ff4623d23b946408fd9cd9ccd2f0e63,
title = "Expression of Le(x) antigen in Schistosoma japonicum and S.haematobium and immune responses to Le(x) in infected animals: Lack of Le(x) expression in other trematodes and nematodes",
abstract = "Adults of the human parasitic trematode Schistosoma mansoni, which causes hepatosplenic/intestinal complications in humans, synthesize glycoconjugates containing the Lewis x (Le(x)) Galβ1→4(Fucα1→3)GlcNAcβ1→R, but not sialyl Lewis x (sLe(x)), antigen. We now report on our analyses of Le(x) and sLe(x) expression in S.haematobium and S.japonicum, which are two other major species of human schistosomes that cause disease, and the possible autoimmunity to these antigens in infected individuals. Antigen expression was evaluated by both ELISA and Western blot analyses of detergent extracts of parasites using monoclonal antibodies. Several high molecular weight glycoproteins in both S.haematobium and S.japonicum contain the Le(x) antigen, but no sialyl Le(x) antigen was detected. In addition, sera from humans and rodents infected with S.haematobium and S.japonicum contain antibodies reactive with Le(x). These results led us to investigate whether Le(x) antigens are expressed in other helminths, including the parasitic trematode Fasciola hepatica, the parasitic nematode Dirofilaria immitis (dog heartworm), the ruminant nematode Haemonchus contortus, and the free-living nematode Caenorhabditis elegans. Neither Le(x) nor sialyl-Le(x) is detectable in these other helminths. Furthermore, none of the helminths, including schistosomes, express Lea, Leb, Le(y), or the H-type 1 antigen. However, several glycoproteins from all helminths analyzed are bound by Lotus tetragonolobus agglutinin, which binds Fucα1→3GlcNAc, and Wisteria floribunda agglutinin, which binds GalNAcβ1→4GlcNAc (lacdiNAc or LDN). Thus, schistosomes may be unique among helminths in expressing the Le(x) antigen, whereas many different helminths may express α1,3-fucosylated glycans and the LDN motif.",
keywords = "Caenorhabditis elegans, Dirofilaria immitis, Fasciola hepatica, Haemonchus contortus, Lewis x antigen, Schistosoma haematobium, Schistosoma japonicum, Schistosoma mansoni",
author = "Nyame, {A. Kwame} and Russell Debose-Boyd and Long, {Troy D.} and Tsang, {Victor C W} and Cummings, {Richard D.}",
year = "1998",
month = "6",
doi = "10.1093/glycob/8.6.615",
language = "English (US)",
volume = "8",
pages = "615--624",
journal = "Glycobiology",
issn = "0959-6658",
publisher = "Oxford University Press",
number = "6",

}

TY - JOUR

T1 - Expression of Le(x) antigen in Schistosoma japonicum and S.haematobium and immune responses to Le(x) in infected animals

T2 - Lack of Le(x) expression in other trematodes and nematodes

AU - Nyame, A. Kwame

AU - Debose-Boyd, Russell

AU - Long, Troy D.

AU - Tsang, Victor C W

AU - Cummings, Richard D.

PY - 1998/6

Y1 - 1998/6

N2 - Adults of the human parasitic trematode Schistosoma mansoni, which causes hepatosplenic/intestinal complications in humans, synthesize glycoconjugates containing the Lewis x (Le(x)) Galβ1→4(Fucα1→3)GlcNAcβ1→R, but not sialyl Lewis x (sLe(x)), antigen. We now report on our analyses of Le(x) and sLe(x) expression in S.haematobium and S.japonicum, which are two other major species of human schistosomes that cause disease, and the possible autoimmunity to these antigens in infected individuals. Antigen expression was evaluated by both ELISA and Western blot analyses of detergent extracts of parasites using monoclonal antibodies. Several high molecular weight glycoproteins in both S.haematobium and S.japonicum contain the Le(x) antigen, but no sialyl Le(x) antigen was detected. In addition, sera from humans and rodents infected with S.haematobium and S.japonicum contain antibodies reactive with Le(x). These results led us to investigate whether Le(x) antigens are expressed in other helminths, including the parasitic trematode Fasciola hepatica, the parasitic nematode Dirofilaria immitis (dog heartworm), the ruminant nematode Haemonchus contortus, and the free-living nematode Caenorhabditis elegans. Neither Le(x) nor sialyl-Le(x) is detectable in these other helminths. Furthermore, none of the helminths, including schistosomes, express Lea, Leb, Le(y), or the H-type 1 antigen. However, several glycoproteins from all helminths analyzed are bound by Lotus tetragonolobus agglutinin, which binds Fucα1→3GlcNAc, and Wisteria floribunda agglutinin, which binds GalNAcβ1→4GlcNAc (lacdiNAc or LDN). Thus, schistosomes may be unique among helminths in expressing the Le(x) antigen, whereas many different helminths may express α1,3-fucosylated glycans and the LDN motif.

AB - Adults of the human parasitic trematode Schistosoma mansoni, which causes hepatosplenic/intestinal complications in humans, synthesize glycoconjugates containing the Lewis x (Le(x)) Galβ1→4(Fucα1→3)GlcNAcβ1→R, but not sialyl Lewis x (sLe(x)), antigen. We now report on our analyses of Le(x) and sLe(x) expression in S.haematobium and S.japonicum, which are two other major species of human schistosomes that cause disease, and the possible autoimmunity to these antigens in infected individuals. Antigen expression was evaluated by both ELISA and Western blot analyses of detergent extracts of parasites using monoclonal antibodies. Several high molecular weight glycoproteins in both S.haematobium and S.japonicum contain the Le(x) antigen, but no sialyl Le(x) antigen was detected. In addition, sera from humans and rodents infected with S.haematobium and S.japonicum contain antibodies reactive with Le(x). These results led us to investigate whether Le(x) antigens are expressed in other helminths, including the parasitic trematode Fasciola hepatica, the parasitic nematode Dirofilaria immitis (dog heartworm), the ruminant nematode Haemonchus contortus, and the free-living nematode Caenorhabditis elegans. Neither Le(x) nor sialyl-Le(x) is detectable in these other helminths. Furthermore, none of the helminths, including schistosomes, express Lea, Leb, Le(y), or the H-type 1 antigen. However, several glycoproteins from all helminths analyzed are bound by Lotus tetragonolobus agglutinin, which binds Fucα1→3GlcNAc, and Wisteria floribunda agglutinin, which binds GalNAcβ1→4GlcNAc (lacdiNAc or LDN). Thus, schistosomes may be unique among helminths in expressing the Le(x) antigen, whereas many different helminths may express α1,3-fucosylated glycans and the LDN motif.

KW - Caenorhabditis elegans

KW - Dirofilaria immitis

KW - Fasciola hepatica

KW - Haemonchus contortus

KW - Lewis x antigen

KW - Schistosoma haematobium

KW - Schistosoma japonicum

KW - Schistosoma mansoni

UR - http://www.scopus.com/inward/record.url?scp=0031597005&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031597005&partnerID=8YFLogxK

U2 - 10.1093/glycob/8.6.615

DO - 10.1093/glycob/8.6.615

M3 - Article

C2 - 9592128

AN - SCOPUS:0031597005

VL - 8

SP - 615

EP - 624

JO - Glycobiology

JF - Glycobiology

SN - 0959-6658

IS - 6

ER -