Expression of LGR8 protein in the reproduction system in insulin-3 knockout mice

Yi Wang, Shao Hua Zhang, Juan Liu, Linda Baker

Research output: Contribution to journalArticle


Objective: Insulin-3 (Insl3) and its only receptor LGR8 (aka RXFP2) are the key mediators in testicular descent. We sought to characterize LGR8 localization in the reproduction system of Insl3 knockout mice to better understand infertility seen in these mice. Methods: IP, Western blot and immunohistochemistry techniques were used to detect the expression of LGR8 protein in the reproduction system of Insl3 knockout mice. Results: LGR8 was found to be expressed in the testis, gubernaculum, epididymis and vas deferens in wildtype mice. The expression of LGR8 varied in different organs, and it was significantly higher in the testis of wildtype mice than in that of Insl3 knockout mice (P<0.05). Conclusion: LGR8 is expressed in the reproduction system of male mice, indicating that Insl3-LGR8 system may play an important role in male infertility.

Original languageEnglish (US)
Pages (from-to)172-175
Number of pages4
JournalJournal of Xi'an Jiaotong University (Medical Sciences)
Issue number2
StatePublished - Mar 1 2012



  • Cryptorchidism
  • Insulin-3
  • Leucine-rich repeat-containing G protein-coupled receptor 8 (LGR8)
  • Male infertility diseases
  • Testicular descent

ASJC Scopus subject areas

  • Clinical Biochemistry

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