Expression of mef2 genes in the mouse central nervous system suggests a role in neuronal maturation

G. E. Lyons, B. K. Micales, J. Schwarz, J. F. Martin, E. N. Olson

Research output: Contribution to journalArticle

209 Citations (Scopus)

Abstract

Members of the myocyte enhancer factor 2 (MEF2) gene family are expressed in a dynamic pattern during development of the CNS of pre- and postnatal mice. The four MEF2 genes, Mef2A, -B, -C, -D, encode transcription factors belonging to the MADS (MCM1-agamous-deficiens-serum response factor) superfamily of DNA binding proteins. MEF2 factors have previously been shown to be positive regulators of gene expression in terminally differentiated muscle cells. To begin to determine the role of MEF2 factors in CNS development, we used in situ hybridization with gene-specific cRNA probes to define the expression patterns of each of the four Mef2 mRNAs in the developing and mature mouse CNS. Mef2C mRNA was first detected in a ventral portion of the telencephalon at 11.5 d postcoitum (p.c.). By 13.5 d p.c., each of the four Mef2 genes were expressed in overlapping yet distinct patterns in regions of the frontal codex, midbrain, thalamus, hippocampus, and hindbrain. Temporal and spatial patterns of embryonic Mef2 gene expression appeared to follow gradients of neuron maturation and suggested that the onset of Mef2 gene expression coincides with withdrawal from the cell cycle and initiation of neuronal differentiation. This correlation is particularly striking for Purkinje cells in the cerebellum. Since the molecular mechanisms that regulate neuron differentiation are unknown, we propose that the MEF2 factors are likely to play an important role in this process.

Original languageEnglish (US)
Pages (from-to)5727-5738
Number of pages12
JournalJournal of Neuroscience
Volume15
Issue number8
StatePublished - 1995

Fingerprint

MEF2 Transcription Factors
Central Nervous System
Gene Expression
Genes
Serum Response Factor
Neurons
Telencephalon
Complementary RNA
Rhombencephalon
Messenger RNA
Purkinje Cells
DNA-Binding Proteins
Regulator Genes
Mesencephalon
Thalamus
Cerebellum
Muscle Cells
In Situ Hybridization
Hippocampus
Cell Cycle

Keywords

  • in situ hybridization
  • Mef2 mRNAs
  • mouse embryo
  • neuron maturation
  • postnatal brain
  • transcription factor genes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of mef2 genes in the mouse central nervous system suggests a role in neuronal maturation. / Lyons, G. E.; Micales, B. K.; Schwarz, J.; Martin, J. F.; Olson, E. N.

In: Journal of Neuroscience, Vol. 15, No. 8, 1995, p. 5727-5738.

Research output: Contribution to journalArticle

Lyons, G. E. ; Micales, B. K. ; Schwarz, J. ; Martin, J. F. ; Olson, E. N. / Expression of mef2 genes in the mouse central nervous system suggests a role in neuronal maturation. In: Journal of Neuroscience. 1995 ; Vol. 15, No. 8. pp. 5727-5738.
@article{79a904577bbf4a8bb042b8a07599f963,
title = "Expression of mef2 genes in the mouse central nervous system suggests a role in neuronal maturation",
abstract = "Members of the myocyte enhancer factor 2 (MEF2) gene family are expressed in a dynamic pattern during development of the CNS of pre- and postnatal mice. The four MEF2 genes, Mef2A, -B, -C, -D, encode transcription factors belonging to the MADS (MCM1-agamous-deficiens-serum response factor) superfamily of DNA binding proteins. MEF2 factors have previously been shown to be positive regulators of gene expression in terminally differentiated muscle cells. To begin to determine the role of MEF2 factors in CNS development, we used in situ hybridization with gene-specific cRNA probes to define the expression patterns of each of the four Mef2 mRNAs in the developing and mature mouse CNS. Mef2C mRNA was first detected in a ventral portion of the telencephalon at 11.5 d postcoitum (p.c.). By 13.5 d p.c., each of the four Mef2 genes were expressed in overlapping yet distinct patterns in regions of the frontal codex, midbrain, thalamus, hippocampus, and hindbrain. Temporal and spatial patterns of embryonic Mef2 gene expression appeared to follow gradients of neuron maturation and suggested that the onset of Mef2 gene expression coincides with withdrawal from the cell cycle and initiation of neuronal differentiation. This correlation is particularly striking for Purkinje cells in the cerebellum. Since the molecular mechanisms that regulate neuron differentiation are unknown, we propose that the MEF2 factors are likely to play an important role in this process.",
keywords = "in situ hybridization, Mef2 mRNAs, mouse embryo, neuron maturation, postnatal brain, transcription factor genes",
author = "Lyons, {G. E.} and Micales, {B. K.} and J. Schwarz and Martin, {J. F.} and Olson, {E. N.}",
year = "1995",
language = "English (US)",
volume = "15",
pages = "5727--5738",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "8",

}

TY - JOUR

T1 - Expression of mef2 genes in the mouse central nervous system suggests a role in neuronal maturation

AU - Lyons, G. E.

AU - Micales, B. K.

AU - Schwarz, J.

AU - Martin, J. F.

AU - Olson, E. N.

PY - 1995

Y1 - 1995

N2 - Members of the myocyte enhancer factor 2 (MEF2) gene family are expressed in a dynamic pattern during development of the CNS of pre- and postnatal mice. The four MEF2 genes, Mef2A, -B, -C, -D, encode transcription factors belonging to the MADS (MCM1-agamous-deficiens-serum response factor) superfamily of DNA binding proteins. MEF2 factors have previously been shown to be positive regulators of gene expression in terminally differentiated muscle cells. To begin to determine the role of MEF2 factors in CNS development, we used in situ hybridization with gene-specific cRNA probes to define the expression patterns of each of the four Mef2 mRNAs in the developing and mature mouse CNS. Mef2C mRNA was first detected in a ventral portion of the telencephalon at 11.5 d postcoitum (p.c.). By 13.5 d p.c., each of the four Mef2 genes were expressed in overlapping yet distinct patterns in regions of the frontal codex, midbrain, thalamus, hippocampus, and hindbrain. Temporal and spatial patterns of embryonic Mef2 gene expression appeared to follow gradients of neuron maturation and suggested that the onset of Mef2 gene expression coincides with withdrawal from the cell cycle and initiation of neuronal differentiation. This correlation is particularly striking for Purkinje cells in the cerebellum. Since the molecular mechanisms that regulate neuron differentiation are unknown, we propose that the MEF2 factors are likely to play an important role in this process.

AB - Members of the myocyte enhancer factor 2 (MEF2) gene family are expressed in a dynamic pattern during development of the CNS of pre- and postnatal mice. The four MEF2 genes, Mef2A, -B, -C, -D, encode transcription factors belonging to the MADS (MCM1-agamous-deficiens-serum response factor) superfamily of DNA binding proteins. MEF2 factors have previously been shown to be positive regulators of gene expression in terminally differentiated muscle cells. To begin to determine the role of MEF2 factors in CNS development, we used in situ hybridization with gene-specific cRNA probes to define the expression patterns of each of the four Mef2 mRNAs in the developing and mature mouse CNS. Mef2C mRNA was first detected in a ventral portion of the telencephalon at 11.5 d postcoitum (p.c.). By 13.5 d p.c., each of the four Mef2 genes were expressed in overlapping yet distinct patterns in regions of the frontal codex, midbrain, thalamus, hippocampus, and hindbrain. Temporal and spatial patterns of embryonic Mef2 gene expression appeared to follow gradients of neuron maturation and suggested that the onset of Mef2 gene expression coincides with withdrawal from the cell cycle and initiation of neuronal differentiation. This correlation is particularly striking for Purkinje cells in the cerebellum. Since the molecular mechanisms that regulate neuron differentiation are unknown, we propose that the MEF2 factors are likely to play an important role in this process.

KW - in situ hybridization

KW - Mef2 mRNAs

KW - mouse embryo

KW - neuron maturation

KW - postnatal brain

KW - transcription factor genes

UR - http://www.scopus.com/inward/record.url?scp=0029085629&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029085629&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 5727

EP - 5738

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 8

ER -