Expression of Na+/Ca2+ exchanger (NCX1) gene in the developmental mouse embryo and adult mouse brain

Koji Wakimoto, Makoto Kuro-o, NoriyGBRi Yanaka, Issei Komuro, Yo ichi Nabeshima, Yuji Imai

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The Na+/Ca2+ exchanger gene, NCX1, is widely expressed in many tissues, encoding several isoforms through alternative RNA splicing. NCX1 deficient mice are known to be lethal at embryonic day 9-10 (E9-10). However, its expression pattern during embryogenesis is largely unknown. Therefore, to identify and compare the localization and alternatively spliced isoforms of NCX1 mRNA expressed in the developmental stages, we analyzed the mouse embryo. Northern blot analysis demonstrated that NCX1 mRNA was expressed from the earliest stage examined, E7. In situ hybridization analysis revealed that NCX1 mRNA was expressed in the heart alone until E10.5. However, at E14.5 and 16.5, NCX1 mRNA was expressed not only in the heart, but also in neuronal cells. In addition, the expression of NCX1 mRNA in the adult brain was most abundant in the hippocampus. Using reverse transcription-polymerase chain reaction (RT-PCR), we also identified the alternatively spliced isoforms expressed during each developmental stage. The restricted expression of the NCX1 gene suggested that NCX1 may play an important role in the developing mouse embryo.

Original languageEnglish (US)
Pages (from-to)191-198
Number of pages8
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume130
Issue number2
DOIs
StatePublished - 2001

Fingerprint

Developmental Genes
Brain
Embryonic Structures
Genes
Messenger RNA
Protein Isoforms
RNA Isoforms
Alternative Splicing
Northern Blotting
Reverse Transcription
Polymerase chain reaction
Embryonic Development
In Situ Hybridization
Hippocampus
Transcription
Gene Expression
Polymerase Chain Reaction
Tissue

Keywords

  • Brain
  • Development
  • Embryo
  • Exchanger
  • Heart
  • Isoform
  • Mouse
  • NCX

ASJC Scopus subject areas

  • Biochemistry
  • Physiology

Cite this

Expression of Na+/Ca2+ exchanger (NCX1) gene in the developmental mouse embryo and adult mouse brain. / Wakimoto, Koji; Kuro-o, Makoto; Yanaka, NoriyGBRi; Komuro, Issei; Nabeshima, Yo ichi; Imai, Yuji.

In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 130, No. 2, 2001, p. 191-198.

Research output: Contribution to journalArticle

Wakimoto, Koji ; Kuro-o, Makoto ; Yanaka, NoriyGBRi ; Komuro, Issei ; Nabeshima, Yo ichi ; Imai, Yuji. / Expression of Na+/Ca2+ exchanger (NCX1) gene in the developmental mouse embryo and adult mouse brain. In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. 2001 ; Vol. 130, No. 2. pp. 191-198.
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