Expression of NHE-3 in the apical membrane of rat renal proximal tubule and thick ascending limb

M. Amemiya, J. Loffing, M. Lotscher, B. Kaissling, R. J. Alpern, O. W. Moe

Research output: Contribution to journalArticle

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Abstract

Apical membrane Na/H exchange is a principal mechanism of renal proximal tubule Na absorption and H secretion, and thick ascending limb H secretion. Based on current data on Na/H exchanger isoforms (NHE-1 to 5), NHE-3 is the likeliest candidate for the apical membrane isoform. The present study localizes NHE-3 in rat kidney using polyclonal antisera against cytoplasmic epitopes of rat NHE-3. These antisera recognized an ~87 kD protein in Na/H exchanger-deficient cells transfected with the rat NHE-3 gene but not in mock-transfected cells. All antisera labeled an ~87 kD protein in plasma membranes from cortex and outer medulla. Fractionation of cortical membranes showed labeling in apical but not basolateral membranes. Cross linking studies suggested existence of oligomeric forms of the transporter. Immunohistochemistry showed strong staining of the apical membrane of S1 convoluted, and S2 convoluted tubule with lesser staining of the S2 straight tubule and absent staining of S3. Weak staining was observed in thin descending limbs in the inner stripe and intense staining was seen in the apical membrane of medullary and cortical thick ascending limbs. NHE-3 staining was absent in the remainder of the nephron. In summary, NHE-3 is the isoform responsible for NaCl and NaHCO3 absorption in the proximal convoluted tubule, and NaHCO3 absorption in the thick ascending limb. In the S3 proximal tubule and the distal convoluted tubule, apical membrane Na/H exchange activity is likely mediated by other isoform(s) of the NHE family.

Original languageEnglish (US)
Pages (from-to)1206-1215
Number of pages10
JournalKidney International
Volume48
Issue number4
StatePublished - 1995

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Proximal Kidney Tubule
Extremities
Staining and Labeling
Membranes
Protein Isoforms
Immune Sera
Sodium-Hydrogen Antiporter
Nephrons
Epitopes
Proteins
Immunohistochemistry
Cell Membrane
Kidney
Genes

ASJC Scopus subject areas

  • Nephrology

Cite this

Amemiya, M., Loffing, J., Lotscher, M., Kaissling, B., Alpern, R. J., & Moe, O. W. (1995). Expression of NHE-3 in the apical membrane of rat renal proximal tubule and thick ascending limb. Kidney International, 48(4), 1206-1215.

Expression of NHE-3 in the apical membrane of rat renal proximal tubule and thick ascending limb. / Amemiya, M.; Loffing, J.; Lotscher, M.; Kaissling, B.; Alpern, R. J.; Moe, O. W.

In: Kidney International, Vol. 48, No. 4, 1995, p. 1206-1215.

Research output: Contribution to journalArticle

Amemiya, M, Loffing, J, Lotscher, M, Kaissling, B, Alpern, RJ & Moe, OW 1995, 'Expression of NHE-3 in the apical membrane of rat renal proximal tubule and thick ascending limb', Kidney International, vol. 48, no. 4, pp. 1206-1215.
Amemiya M, Loffing J, Lotscher M, Kaissling B, Alpern RJ, Moe OW. Expression of NHE-3 in the apical membrane of rat renal proximal tubule and thick ascending limb. Kidney International. 1995;48(4):1206-1215.
Amemiya, M. ; Loffing, J. ; Lotscher, M. ; Kaissling, B. ; Alpern, R. J. ; Moe, O. W. / Expression of NHE-3 in the apical membrane of rat renal proximal tubule and thick ascending limb. In: Kidney International. 1995 ; Vol. 48, No. 4. pp. 1206-1215.
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