Expression of RCAS1 and its function in human squamous cell carcinoma of the oral cavity

Masakatsu Fukuda, Akio Tanaka, A. Y A Hamao, Seiji Suzuki, Kaoru Kusama, Hideaki Sakashita

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) is a recently discovered human tumor-associated antigen expressed in a wide variety of cancer tissues. It has been suggested that tumor cells evade from immune surveillance by expression of RCAS1. We investigated whether RCAS1 is expressed in human oral squamous cell carcinomas (HOSCCs) or HOSCC (HSC-2, -3, -4, Ca9-22 and KB) cells and whether tumor cells expressing RCAS1, induce apoptosis in its receptor-positive cells, peripheral blood lymphocytes (PBLs). RCAS1 transcripts and proteins were detected in five HOSCC cell types. Immunohistochemical examinations revealed that RCAS1 was slightly to moderately positive in 32 of 40 cases (80%) of HOSCCs. Furthermore, the frequency of RCAS1 expression in HOSCC increased with advancing tumor stage according to the pTNM system. Confocal laser microscopy and DNA fragmentation assay showed that PBLs, which were stimulated with IL-2, underwent apoptosis by co-culturing with KB cells. It was also confirmed by Western dot blotting that soluble RCAS1 is secreted into culture supernatants of KB cells. The apoptotic tumor-infiltrating lymphocytes (TILs) were detected around RCAS1-positive tumor cells in HOSCCs by TUNEL method. These results suggest that RCAS1 expression might be associated with progression of oral tumors and offer a possible mechanism for oral cancer immune escape.

Original languageEnglish (US)
Pages (from-to)259-267
Number of pages9
JournalOncology Reports
Volume12
Issue number2
StatePublished - Aug 2004

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Mouth
Squamous Cell Carcinoma
KB Cells
Neoplasms
Confocal Microscopy
Lymphocytes
Apoptosis
Tumor-Infiltrating Lymphocytes
Mouth Neoplasms
In Situ Nick-End Labeling
Neoplasm Antigens
DNA Fragmentation
Interleukin-2
Blood Cells
Western Blotting
Antigens
Proteins

Keywords

  • Confocal laser microscope
  • Human oral squamous cell carcinoma (HOSCC)
  • Immune escape
  • Immunohistochemistry
  • Receptor-binding cancer antigen expressed on siso cells (RCAS1)

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Fukuda, M., Tanaka, A., Hamao, A. Y. A., Suzuki, S., Kusama, K., & Sakashita, H. (2004). Expression of RCAS1 and its function in human squamous cell carcinoma of the oral cavity. Oncology Reports, 12(2), 259-267.

Expression of RCAS1 and its function in human squamous cell carcinoma of the oral cavity. / Fukuda, Masakatsu; Tanaka, Akio; Hamao, A. Y A; Suzuki, Seiji; Kusama, Kaoru; Sakashita, Hideaki.

In: Oncology Reports, Vol. 12, No. 2, 08.2004, p. 259-267.

Research output: Contribution to journalArticle

Fukuda, M, Tanaka, A, Hamao, AYA, Suzuki, S, Kusama, K & Sakashita, H 2004, 'Expression of RCAS1 and its function in human squamous cell carcinoma of the oral cavity', Oncology Reports, vol. 12, no. 2, pp. 259-267.
Fukuda M, Tanaka A, Hamao AYA, Suzuki S, Kusama K, Sakashita H. Expression of RCAS1 and its function in human squamous cell carcinoma of the oral cavity. Oncology Reports. 2004 Aug;12(2):259-267.
Fukuda, Masakatsu ; Tanaka, Akio ; Hamao, A. Y A ; Suzuki, Seiji ; Kusama, Kaoru ; Sakashita, Hideaki. / Expression of RCAS1 and its function in human squamous cell carcinoma of the oral cavity. In: Oncology Reports. 2004 ; Vol. 12, No. 2. pp. 259-267.
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