Expression of reg/PSP, a pancreatic exocrine gene: Relationship to changes in islet β-cell mass

Chisato Miyaura, Ling Chen, Michael Appel, Tausif Alam, Lindsey Inman, Steven D. Hughes, Joseph Leslie Milburn Jr., Roger H Unger, Christopher B. Newgard

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Abstract

A cDNA termed reg was recently isolated by differential screening of a library prepared from regenerating islets isolated from pancreatic remnants of rats subjected to 90% pancreatectomy and nicotinamide treatment. This led to speculation that this gene may be involved in expansion of β-cell mass. In the current study we have measured reg expression after implantation and resection of a solid insulinoma tumor into rats, manuevers known, respectively, to reduce and reexpand the volume of β-cells in the islet. Animals with an implanted insulinoma tumor became profoundly hypoglycemic. Islet β-cells declined from the normal 75% of total islet volume to less than 30%, in concert with a marked reduction in the reg mRNA level. Removal of the tumor resulted in a sharp increase in β-cell replication, as measured by [3H]thymidine incorporation and a return to normal β-cell volume within 4 days of tumor resection. This was associated with a transient induction in reg expression compared to that in tumor-bearing animals, effectively returning the amount of reg mRNA to the levels found in normal animals within 48 h; at later time points after tumor removal (3-7 days) reg expression declined, but then rose toward normal. In situ hybridization analysis localized the initial induction in reg mRNA expression to the exocrine pancreas. Continuous infusion of insulin into normal rats for 4 days, a manuever that does not significantly reduce β-cell mass, resulted in dramatically reduced insulin mRNA in islets, but no change in the levels of reg mRNA. We conclude that the diminution in pancreatic β-cell mass caused by subcutaneous implantation of an insulinoma is associated with reduced reg gene expression and that the increase in β-cell replication after resection of the tumor is preceded by return of reg gene expression toward normal.

Original languageEnglish (US)
Pages (from-to)226-234
Number of pages9
JournalMolecular Endocrinology
Volume5
Issue number2
StatePublished - Feb 1991

Fingerprint

Islets of Langerhans
Insulinoma
Genes
Messenger RNA
Neoplasms
Cell Size
Insulin
Gene Expression
Exocrine Pancreas
Pancreatectomy
Niacinamide
Primary Spontaneous Pneumothorax
Hypoglycemic Agents
Thymidine
Libraries
In Situ Hybridization
Complementary DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Miyaura, C., Chen, L., Appel, M., Alam, T., Inman, L., Hughes, S. D., ... Newgard, C. B. (1991). Expression of reg/PSP, a pancreatic exocrine gene: Relationship to changes in islet β-cell mass. Molecular Endocrinology, 5(2), 226-234.

Expression of reg/PSP, a pancreatic exocrine gene : Relationship to changes in islet β-cell mass. / Miyaura, Chisato; Chen, Ling; Appel, Michael; Alam, Tausif; Inman, Lindsey; Hughes, Steven D.; Milburn Jr., Joseph Leslie; Unger, Roger H; Newgard, Christopher B.

In: Molecular Endocrinology, Vol. 5, No. 2, 02.1991, p. 226-234.

Research output: Contribution to journalArticle

Miyaura, C, Chen, L, Appel, M, Alam, T, Inman, L, Hughes, SD, Milburn Jr., JL, Unger, RH & Newgard, CB 1991, 'Expression of reg/PSP, a pancreatic exocrine gene: Relationship to changes in islet β-cell mass', Molecular Endocrinology, vol. 5, no. 2, pp. 226-234.
Miyaura C, Chen L, Appel M, Alam T, Inman L, Hughes SD et al. Expression of reg/PSP, a pancreatic exocrine gene: Relationship to changes in islet β-cell mass. Molecular Endocrinology. 1991 Feb;5(2):226-234.
Miyaura, Chisato ; Chen, Ling ; Appel, Michael ; Alam, Tausif ; Inman, Lindsey ; Hughes, Steven D. ; Milburn Jr., Joseph Leslie ; Unger, Roger H ; Newgard, Christopher B. / Expression of reg/PSP, a pancreatic exocrine gene : Relationship to changes in islet β-cell mass. In: Molecular Endocrinology. 1991 ; Vol. 5, No. 2. pp. 226-234.
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