Expression of ricin A chain in Escherichia coli

Mary O'Hare, Lynne M. Roberts, Philip E. Thorpe, Graham J. Watson, Bernadette Prior, J. Michael Lord

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

DNA encoding ricin A chain was derived from preproricin cDNA and ligated into the expression vector pDS5/3. Transcription is controlled from the coliphage promoter PN25 fused with the lac operator of E.coli. When induced, E.coli 71.18 cells transformed with the recombinant plasmid express ricin A chain which is soluble and has full biological activity.

Original languageEnglish (US)
Pages (from-to)73-78
Number of pages6
JournalFEBS Letters
Volume216
Issue number1
DOIs
StatePublished - May 25 1987

Fingerprint

Ricin
Escherichia coli
Coliphages
Transcription
Bioactivity
Plasmids
Complementary DNA
DNA
preproricin

Keywords

  • (E.coli)
  • Expression
  • Immunotoxin
  • Ricin A chain

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

O'Hare, M., Roberts, L. M., Thorpe, P. E., Watson, G. J., Prior, B., & Lord, J. M. (1987). Expression of ricin A chain in Escherichia coli. FEBS Letters, 216(1), 73-78. https://doi.org/10.1016/0014-5793(87)80759-7

Expression of ricin A chain in Escherichia coli. / O'Hare, Mary; Roberts, Lynne M.; Thorpe, Philip E.; Watson, Graham J.; Prior, Bernadette; Lord, J. Michael.

In: FEBS Letters, Vol. 216, No. 1, 25.05.1987, p. 73-78.

Research output: Contribution to journalArticle

O'Hare, M, Roberts, LM, Thorpe, PE, Watson, GJ, Prior, B & Lord, JM 1987, 'Expression of ricin A chain in Escherichia coli', FEBS Letters, vol. 216, no. 1, pp. 73-78. https://doi.org/10.1016/0014-5793(87)80759-7
O'Hare M, Roberts LM, Thorpe PE, Watson GJ, Prior B, Lord JM. Expression of ricin A chain in Escherichia coli. FEBS Letters. 1987 May 25;216(1):73-78. https://doi.org/10.1016/0014-5793(87)80759-7
O'Hare, Mary ; Roberts, Lynne M. ; Thorpe, Philip E. ; Watson, Graham J. ; Prior, Bernadette ; Lord, J. Michael. / Expression of ricin A chain in Escherichia coli. In: FEBS Letters. 1987 ; Vol. 216, No. 1. pp. 73-78.
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