Expression of ricin A chain in Escherichia coli

Mary O'Hare, Lynne M. Roberts, Philip E. Thorpe, Graham J. Watson, Bernadette Prior, J. Michael Lord

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

DNA encoding ricin A chain was derived from preproricin cDNA and ligated into the expression vector pDS5/3. Transcription is controlled from the coliphage promoter PN25 fused with the lac operator of E.coli. When induced, E.coli 71.18 cells transformed with the recombinant plasmid express ricin A chain which is soluble and has full biological activity.

Original languageEnglish (US)
Pages (from-to)73-78
Number of pages6
JournalFEBS Letters
Volume216
Issue number1
DOIs
StatePublished - May 25 1987

Keywords

  • (E.coli)
  • Expression
  • Immunotoxin
  • Ricin A chain

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    O'Hare, M., Roberts, L. M., Thorpe, P. E., Watson, G. J., Prior, B., & Lord, J. M. (1987). Expression of ricin A chain in Escherichia coli. FEBS Letters, 216(1), 73-78. https://doi.org/10.1016/0014-5793(87)80759-7