Expression of ricin B chain in Spodoptera frugiperda

A. Frankel, H. Roberts, L. Afrin, J. Vesely, M. Willingham

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

DNA encoding ricin B chain was derived from preproricin genomic DNA and ligated into the baculovirus transfer vector, pAcGP67A. Co-transfection of Spodoptera frugiperda Sf9 cells with BaculoGold DNA was followed by limiting dilution purification of recombinant baculovirus. Infection of SF9 cells at a multiplicity of infection of 1 in the presence of 25 mM lactose produced 3 mg/l of soluble, glycosylated, 34 kDa protein immunoreactive with monoclonal and polyclonal antibodies to ricin B chain. The recombinant ricin B chain had similar lectin-binding activity to plant ricin B chain. The recombinant protein reassociated with ricin toxin A chain similarly to ricin toxin B chain and the recombinant heterodimers had similar cell cytotoxicity to ricin.

Original languageEnglish (US)
Pages (from-to)787-794
Number of pages8
JournalBiochemical Journal
Volume303
Issue number3
StatePublished - 1994

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Ricin
Spodoptera
Baculoviridae
DNA
Sf9 Cells
Lactose
Cytotoxicity
Infection
Recombinant Proteins
Lectins
Dilution
Purification
Transfection
Monoclonal Antibodies
Antibodies

ASJC Scopus subject areas

  • Biochemistry

Cite this

Frankel, A., Roberts, H., Afrin, L., Vesely, J., & Willingham, M. (1994). Expression of ricin B chain in Spodoptera frugiperda. Biochemical Journal, 303(3), 787-794.

Expression of ricin B chain in Spodoptera frugiperda. / Frankel, A.; Roberts, H.; Afrin, L.; Vesely, J.; Willingham, M.

In: Biochemical Journal, Vol. 303, No. 3, 1994, p. 787-794.

Research output: Contribution to journalArticle

Frankel, A, Roberts, H, Afrin, L, Vesely, J & Willingham, M 1994, 'Expression of ricin B chain in Spodoptera frugiperda', Biochemical Journal, vol. 303, no. 3, pp. 787-794.
Frankel A, Roberts H, Afrin L, Vesely J, Willingham M. Expression of ricin B chain in Spodoptera frugiperda. Biochemical Journal. 1994;303(3):787-794.
Frankel, A. ; Roberts, H. ; Afrin, L. ; Vesely, J. ; Willingham, M. / Expression of ricin B chain in Spodoptera frugiperda. In: Biochemical Journal. 1994 ; Vol. 303, No. 3. pp. 787-794.
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