TY - JOUR
T1 - Expression of the chicken transferrin gene in transgenic mice
AU - Stanley McKnight, G.
AU - Hammer, Robert E
AU - Kuenzel, Elizabeth A.
AU - Brinster, Ralph L.
N1 - Funding Information:
We gratefully acknowledge Pierre Chambon for sending us the plasmid containrng the transferrin gene. We thank Myma Trumbauer for superb technical assistance, and we appreciate constructive comments of David Lee, Michel Sanders, and David Carmichael on the preparation of the manuscript. We are also grateful to Joe Beavo for advice on the measurement of serum transferrin by radioimmunoassay. The work was supported by grants from the National Institutes of Health and the National Science Foundation.
PY - 1983/9
Y1 - 1983/9
N2 - The chicken transferrin gene was microinjected into the male pronucleus of fertilized mouse eggs, and the eggs were then implanted into foster mothers. Approximately 15%-30% of the offspring from the injected eggs carried chicken DNA sequences; restriction mapping indicated that multiple copies of the chicken gene had integrated into the genome in a tandem arrangement in most of the mice. Six of the seven mice studied expressed the chicken gene, and in five mice there was a 5 to 10 fold preferential expression of chicken transferrin mRNA in liver compared to that in other tissues. Chicken transferrin was secreted into the serum of five of the mice, where it reached steady state concentrations up to 67 μg/ml. Offspring from transgenic parents also expressed the chicken gene; in some cases the expression in offspring was very similar to the parent, but in one line expression in offspring had increased 2 to 4 fold.
AB - The chicken transferrin gene was microinjected into the male pronucleus of fertilized mouse eggs, and the eggs were then implanted into foster mothers. Approximately 15%-30% of the offspring from the injected eggs carried chicken DNA sequences; restriction mapping indicated that multiple copies of the chicken gene had integrated into the genome in a tandem arrangement in most of the mice. Six of the seven mice studied expressed the chicken gene, and in five mice there was a 5 to 10 fold preferential expression of chicken transferrin mRNA in liver compared to that in other tissues. Chicken transferrin was secreted into the serum of five of the mice, where it reached steady state concentrations up to 67 μg/ml. Offspring from transgenic parents also expressed the chicken gene; in some cases the expression in offspring was very similar to the parent, but in one line expression in offspring had increased 2 to 4 fold.
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U2 - 10.1016/0092-8674(83)90368-9
DO - 10.1016/0092-8674(83)90368-9
M3 - Article
C2 - 6311428
AN - SCOPUS:0020562991
SN - 0092-8674
VL - 34
SP - 335
EP - 341
JO - Cell
JF - Cell
IS - 2
ER -