Expression of the chicken transferrin gene in transgenic mice

G. Stanley McKnight, Robert E Hammer, Elizabeth A. Kuenzel, Ralph L. Brinster

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

The chicken transferrin gene was microinjected into the male pronucleus of fertilized mouse eggs, and the eggs were then implanted into foster mothers. Approximately 15%-30% of the offspring from the injected eggs carried chicken DNA sequences; restriction mapping indicated that multiple copies of the chicken gene had integrated into the genome in a tandem arrangement in most of the mice. Six of the seven mice studied expressed the chicken gene, and in five mice there was a 5 to 10 fold preferential expression of chicken transferrin mRNA in liver compared to that in other tissues. Chicken transferrin was secreted into the serum of five of the mice, where it reached steady state concentrations up to 67 μg/ml. Offspring from transgenic parents also expressed the chicken gene; in some cases the expression in offspring was very similar to the parent, but in one line expression in offspring had increased 2 to 4 fold.

Original languageEnglish (US)
Pages (from-to)335-341
Number of pages7
JournalCell
Volume34
Issue number2
DOIs
StatePublished - Sep 1983

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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