Expression of the common cytokine receptor γ chain by murine dendritic cells including epidermal Langerhans cells

Mansour Mohamadzadeh, Kiyoshi Ariizumi, Kazuo Sugamura, Paul R. Bergstresser, Akira Takashima

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The common cytokine receptor γ chain (γ(c)) is an indispensable component of interleukin (IL)-2, IL-4, IL-7, IL-9, and IL-15 receptors, and its expression has been detected in several leukocyte populations, including T cells, B cells, monocytes, natural killer cells, and neutrophils. The purpose of this study was to determine whether γ receptors are expressed by dendritic cells (DC). Constitutive γ(c) mRNA expression was observed by reverse transcription polymerase chain reaction and/or Northern blotting for: (a) Ia+ epidermal Langerhans cells (LC), (b) 4F7+ splenic DC, (c) granulocyte/macrophage colony-stimulated factor-propagated bone marrow-derived DC, and (d) the epidermal-derived DC line, XS52, which retains important functions of epidermal LC. Exposure of XS52 cells to recombinant IL-4 induced a rapid up-regulation of c-myc mRNA expression, and this IL-4-dependent signaling was blocked almost completely by anti-γ(c) monoclonal antibody (mAb) TUGm2 in a soluble form. Moreover, c-myc up-regulation was inducible in XS52 cells by the same mAb in an immobilized form. These results imply that molecules recognized by this antibody (i.e. γ(c) receptors) are expressed on XS52 cell surfaces. We thus conclude that DC express functional γ(c) receptors, which then mediate cytokine-dependent regulation of DC functions.

Original languageEnglish (US)
Pages (from-to)156-160
Number of pages5
JournalEuropean Journal of Immunology
Volume26
Issue number1
DOIs
StatePublished - Jan 1996

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Cytokine Receptors
Langerhans Cells
Dendritic Cells
Interleukin-4
Interleukin-15 Receptors
Up-Regulation
Monoclonal Antibodies
Interleukin-9
Interleukin-7
Messenger RNA
Granulocytes
Natural Killer Cells
Northern Blotting
Reverse Transcription
Interleukin-2
Monocytes
Neutrophils
Leukocytes
B-Lymphocytes
Bone Marrow

Keywords

  • γ(c) chain
  • Dendritic cell
  • Interleukin-2 receptor
  • Langerhans cells

ASJC Scopus subject areas

  • Immunology

Cite this

Expression of the common cytokine receptor γ chain by murine dendritic cells including epidermal Langerhans cells. / Mohamadzadeh, Mansour; Ariizumi, Kiyoshi; Sugamura, Kazuo; Bergstresser, Paul R.; Takashima, Akira.

In: European Journal of Immunology, Vol. 26, No. 1, 01.1996, p. 156-160.

Research output: Contribution to journalArticle

Mohamadzadeh, Mansour ; Ariizumi, Kiyoshi ; Sugamura, Kazuo ; Bergstresser, Paul R. ; Takashima, Akira. / Expression of the common cytokine receptor γ chain by murine dendritic cells including epidermal Langerhans cells. In: European Journal of Immunology. 1996 ; Vol. 26, No. 1. pp. 156-160.
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