Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi 3 T in Escherichia coli

S. D. Ehrlich, H. Bursztyn Pettegrew, I. Stroynowski, J. Lederberg

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The thymidylate synthetase gene of B. subtilis bacteriophage Phi 3 T, when cloned in plasmids pSC101 or pMB9, is expressed in E. coli. The promoter of the cloned gene is likely to originate in Phi 3 T. Rearrangements of hybrid plasmid sequences during the cloning have been noted. B. subtilis strains can be transformed with hybrid DNAs. The transformants contain sequences of Phi 3 T, but not those of plasmid vectors.

Original languageEnglish (US)
Pages (from-to)4145-4149
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume73
Issue number11
StatePublished - 1976

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Bacillus Phages
Thymidylate Synthase
Bacillus subtilis
Plasmids
Escherichia coli
Genes
Organism Cloning
DNA

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi 3 T in Escherichia coli. / Ehrlich, S. D.; Bursztyn Pettegrew, H.; Stroynowski, I.; Lederberg, J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 73, No. 11, 1976, p. 4145-4149.

Research output: Contribution to journalArticle

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