Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi 3 T in Escherichia coli

S. D. Ehrlich, H. Bursztyn Pettegrew, I. Stroynowski, J. Lederberg

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The thymidylate synthetase gene of B. subtilis bacteriophage Phi 3 T, when cloned in plasmids pSC101 or pMB9, is expressed in E. coli. The promoter of the cloned gene is likely to originate in Phi 3 T. Rearrangements of hybrid plasmid sequences during the cloning have been noted. B. subtilis strains can be transformed with hybrid DNAs. The transformants contain sequences of Phi 3 T, but not those of plasmid vectors.

Original languageEnglish (US)
Pages (from-to)4145-4149
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number11
StatePublished - Jan 1 1976


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