TY - JOUR
T1 - Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi 3 T in Escherichia coli
AU - Ehrlich, S. D.
AU - Bursztyn Pettegrew, H.
AU - Stroynowski, I.
AU - Lederberg, J.
PY - 1976
Y1 - 1976
N2 - The thymidylate synthetase gene of B. subtilis bacteriophage Phi 3 T, when cloned in plasmids pSC101 or pMB9, is expressed in E. coli. The promoter of the cloned gene is likely to originate in Phi 3 T. Rearrangements of hybrid plasmid sequences during the cloning have been noted. B. subtilis strains can be transformed with hybrid DNAs. The transformants contain sequences of Phi 3 T, but not those of plasmid vectors.
AB - The thymidylate synthetase gene of B. subtilis bacteriophage Phi 3 T, when cloned in plasmids pSC101 or pMB9, is expressed in E. coli. The promoter of the cloned gene is likely to originate in Phi 3 T. Rearrangements of hybrid plasmid sequences during the cloning have been noted. B. subtilis strains can be transformed with hybrid DNAs. The transformants contain sequences of Phi 3 T, but not those of plasmid vectors.
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U2 - 10.1073/pnas.73.11.4145
DO - 10.1073/pnas.73.11.4145
M3 - Article
C2 - 825858
AN - SCOPUS:0017131803
SN - 0027-8424
VL - 73
SP - 4145
EP - 4149
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 11
ER -