The thymidylate synthetase gene of B. subtilis bacteriophage Phi 3 T, when cloned in plasmids pSC101 or pMB9, is expressed in E. coli. The promoter of the cloned gene is likely to originate in Phi 3 T. Rearrangements of hybrid plasmid sequences during the cloning have been noted. B. subtilis strains can be transformed with hybrid DNAs. The transformants contain sequences of Phi 3 T, but not those of plasmid vectors.
|Original language||English (US)|
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - Jan 1 1976|
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