Expression of thyrotropin-releasing hormone receptors by adenovirus-mediated gene transfer reveals that thyrotropin-releasing hormone desensitization is cell specific

Erik Falck-Pedersen, Marcos Heinflink, Mauricio Alvira, Daniel R. Nussenzveig, Marvin C. Gershengorn

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Biological studies of seven-transmembrane region G protein-coupled receptors have been restricted by available techniques for gene transfer into mammalian cells. We have created a highly efficient adenovirus-based expression vector for the thyrotropin-releasing hormone (TRH) receptor (TRH-R), AdCMVmTRHR, to circumvent difficulties encountered when transient or stable plasmid expression systems are used. We show that infection with AdCMVmTRHR results in fully functional TRH-Rs, which can be expressed in a broad range of mammalian cell types, including those resistant to conventional transient transfection. TRH-Rs can be expressed at high levels, up to 2 × 106 receptors/cell. Expression in several cell lines in culture reveals that rapid TRH-R desensitization by TRH and phorbol 12-myristate 13-acetate is cell type specific. The versatility of adenovirus-mediated gene transfer and expression of TRH-Rs not only facilitates in vitro studies of TRH-R biology but also provides a valuable in vivo expression vector capable of extending TRH-R studies to animal model systems.

Original languageEnglish (US)
Pages (from-to)684-689
Number of pages6
JournalMolecular Pharmacology
Volume45
Issue number4
StatePublished - Apr 1994

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Thyrotropin Releasing Hormone Receptors
Thyrotropin-Releasing Hormone
Adenoviridae
Genes
Gene Transfer Techniques
Transfection
Acetates
Plasmids
Animal Models
Gene Expression
Cell Line

ASJC Scopus subject areas

  • Pharmacology

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Expression of thyrotropin-releasing hormone receptors by adenovirus-mediated gene transfer reveals that thyrotropin-releasing hormone desensitization is cell specific. / Falck-Pedersen, Erik; Heinflink, Marcos; Alvira, Mauricio; Nussenzveig, Daniel R.; Gershengorn, Marvin C.

In: Molecular Pharmacology, Vol. 45, No. 4, 04.1994, p. 684-689.

Research output: Contribution to journalArticle

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