Expression of transforming growth factors α and β in colonic mucosa in inflammatory bowel disease

M. W. Babyatsky, G. Rossiter, D. K. Podolsky

Research output: Contribution to journalArticle

300 Scopus citations

Abstract

Background and Aims: Transforming growth factors (TGFs) α and β are key regulatory peptides that modulate mucosal cell populations critical to inflammatory bowel disease. The aim of this study was to assess TGF-α and TGF-β expression in human colonic mucosa. Methods: TGF-α and TGF-β expression was assessed in colonic mucosa from patients with ulcerative colitis, patients with Crohn's disease, and controls by Northern blot analysis, in situ hybridization, and bioassay. Results: TGF-α messenger RNA expression localized to the villous tips of the small intestine and the surface epithelium of the colon. TGF-α expression was enhanced 2.3-fold in inactive ulcerative colitis mucosa relative to active ulcerative colitis, Crohn's disease, or normal controls. Enhanced expression correlated with duration of disease. TGF-α expression was increased in affected mucosa from both patients with ulcerative colitis and Crohn's disease with active disease. TGF-β1 messenger RNA expression in ulcerative colitis and Crohn's disease localized mostly to cells of the lamina propria with the highest concentration in inflammatory cells closest to the luminal surface. Conclusions: TGF-α may contribute to epithelial hyperproliferation and the increased risk of malignancy in long-standing ulcerative colitis. TGF-β may be a key cytokine during periods of active inflammation, modulating epithelial cell restitution and functional features of cells within the lamina propria.

Original languageEnglish (US)
Pages (from-to)975-984
Number of pages10
JournalGastroenterology
Volume110
Issue number4
DOIs
StatePublished - Jan 1 1996

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Fingerprint Dive into the research topics of 'Expression of transforming growth factors α and β in colonic mucosa in inflammatory bowel disease'. Together they form a unique fingerprint.

  • Cite this