TY - JOUR
T1 - Expression profiling of nuclear receptors in human and mouse embryonic stem cells
AU - Xie, Chang Qing
AU - Jeong, Yangsik
AU - Fu, Mingui
AU - Bookout, Angie L.
AU - Garcia-Barrio, Minerva T.
AU - Sun, Tingwan
AU - Kim, Bong Hyun
AU - Xie, Yang
AU - Root, Sierra
AU - Zhang, Jifeng
AU - Xu, Ren He
AU - Chen, Y. Eugene
AU - Mangelsdorf, David J.
PY - 2009/5
Y1 - 2009/5
N2 - Nuclear receptors (NRs) regulate gene expression in essential biological processes including differentiation and development. Here we report the systematic profiling of NRs in human and mouse embryonic stem cell (ESC) lines and during their early differentiation into embryoid bodies. Expression of the 48 human and mouse NRs was assessed by quantitative real-time PCR. In general, expression of NRs between the two human cell lines was highly concordant, whereas in contrast, expression of NRs between human and mouse ESCs differed significantly. In particular, a number of NRs that have been implicated previously as crucial regulators of mouse ESC biology, including ERRβ, DAX-1, and LRH-1, exhibited diametric patterns of expression, suggesting they may have distinct species-specific functions. Taken together, these results highlight the complexity of the transcrip- tional hierarchy that exists between species and governs early development. These data should provide a unique resource for further exploration of the species-specific roles of NRs in ESC self-renewal and differentiation.
AB - Nuclear receptors (NRs) regulate gene expression in essential biological processes including differentiation and development. Here we report the systematic profiling of NRs in human and mouse embryonic stem cell (ESC) lines and during their early differentiation into embryoid bodies. Expression of the 48 human and mouse NRs was assessed by quantitative real-time PCR. In general, expression of NRs between the two human cell lines was highly concordant, whereas in contrast, expression of NRs between human and mouse ESCs differed significantly. In particular, a number of NRs that have been implicated previously as crucial regulators of mouse ESC biology, including ERRβ, DAX-1, and LRH-1, exhibited diametric patterns of expression, suggesting they may have distinct species-specific functions. Taken together, these results highlight the complexity of the transcrip- tional hierarchy that exists between species and governs early development. These data should provide a unique resource for further exploration of the species-specific roles of NRs in ESC self-renewal and differentiation.
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U2 - 10.1210/me.2008-0465
DO - 10.1210/me.2008-0465
M3 - Article
C2 - 19196830
AN - SCOPUS:66649100583
SN - 0888-8809
VL - 23
SP - 724
EP - 733
JO - Molecular Endocrinology
JF - Molecular Endocrinology
IS - 5
ER -