Expression proteomics identifies biochemical adaptations and defense responses in transgenic plants with perturbed polyamine metabolism

Marina Franceschetti; Barry Perry; Benjamin Thompson; Colin Hanfrey; Anthony J. Michael

doi:10.1016/j.febslet.2004.08.085

Expression proteomics identifies biochemical adaptations and defense responses in transgenic plants with perturbed polyamine metabolism

Marina Franceschetti, Barry Perry, Benjamin Thompson, Colin Hanfrey, Anthony J. Michael

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Soluble proteins from leaves of transgenic tobacco plants with perturbed polyamine metabolism, caused by S-adenosylmethionine decarboxylase overexpression, were analysed by comparative proteomics. A group of proteins was found to be increasingly repressed, in parallel with the degree of polyamine perturbation, in each of the three independent transgenic lines. These were identified as isoforms of chloroplast ribonucleoproteins, known to be involved in chloroplast mRNA stability, processing and translation. Another group of eight proteins strongly induced in the most metabolically perturbed line was identified as multiple, uncharacterised isoforms of the defense protein PR-1, a known marker for systemic acquired resistance.

Original language	English (US)
Pages (from-to)	477-480
Number of pages	4
Journal	FEBS Letters
Volume	576
Issue number	3
DOIs	https://doi.org/10.1016/j.febslet.2004.08.085
State	Published - Oct 22 2004

Keywords

Chloroplast ribonucleoprotein
PR-1
Polyamine
Proteomic
S-Adenosylmethionine decarboxylase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2004.08.085

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title = "Expression proteomics identifies biochemical adaptations and defense responses in transgenic plants with perturbed polyamine metabolism",

abstract = "Soluble proteins from leaves of transgenic tobacco plants with perturbed polyamine metabolism, caused by S-adenosylmethionine decarboxylase overexpression, were analysed by comparative proteomics. A group of proteins was found to be increasingly repressed, in parallel with the degree of polyamine perturbation, in each of the three independent transgenic lines. These were identified as isoforms of chloroplast ribonucleoproteins, known to be involved in chloroplast mRNA stability, processing and translation. Another group of eight proteins strongly induced in the most metabolically perturbed line was identified as multiple, uncharacterised isoforms of the defense protein PR-1, a known marker for systemic acquired resistance.",

keywords = "Chloroplast ribonucleoprotein, PR-1, Polyamine, Proteomic, S-Adenosylmethionine decarboxylase",

author = "Marina Franceschetti and Barry Perry and Benjamin Thompson and Colin Hanfrey and Michael, {Anthony J.}",

note = "Funding Information: M.F. was supported by an E.U. Marie Curie fellowship (MCFI 2000-02029). We thank Julie Hofer for constructive criticism of the manuscript.",

year = "2004",

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doi = "10.1016/j.febslet.2004.08.085",

language = "English (US)",

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T1 - Expression proteomics identifies biochemical adaptations and defense responses in transgenic plants with perturbed polyamine metabolism

AU - Franceschetti, Marina

AU - Perry, Barry

AU - Thompson, Benjamin

AU - Hanfrey, Colin

AU - Michael, Anthony J.

N1 - Funding Information: M.F. was supported by an E.U. Marie Curie fellowship (MCFI 2000-02029). We thank Julie Hofer for constructive criticism of the manuscript.

PY - 2004/10/22

Y1 - 2004/10/22

N2 - Soluble proteins from leaves of transgenic tobacco plants with perturbed polyamine metabolism, caused by S-adenosylmethionine decarboxylase overexpression, were analysed by comparative proteomics. A group of proteins was found to be increasingly repressed, in parallel with the degree of polyamine perturbation, in each of the three independent transgenic lines. These were identified as isoforms of chloroplast ribonucleoproteins, known to be involved in chloroplast mRNA stability, processing and translation. Another group of eight proteins strongly induced in the most metabolically perturbed line was identified as multiple, uncharacterised isoforms of the defense protein PR-1, a known marker for systemic acquired resistance.

AB - Soluble proteins from leaves of transgenic tobacco plants with perturbed polyamine metabolism, caused by S-adenosylmethionine decarboxylase overexpression, were analysed by comparative proteomics. A group of proteins was found to be increasingly repressed, in parallel with the degree of polyamine perturbation, in each of the three independent transgenic lines. These were identified as isoforms of chloroplast ribonucleoproteins, known to be involved in chloroplast mRNA stability, processing and translation. Another group of eight proteins strongly induced in the most metabolically perturbed line was identified as multiple, uncharacterised isoforms of the defense protein PR-1, a known marker for systemic acquired resistance.

KW - Chloroplast ribonucleoprotein

KW - PR-1

KW - Polyamine

KW - Proteomic

KW - S-Adenosylmethionine decarboxylase

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SN - 0014-5793

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EP - 480

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Expression proteomics identifies biochemical adaptations and defense responses in transgenic plants with perturbed polyamine metabolism

Abstract

Keywords

ASJC Scopus subject areas

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