Expression proteomics identifies biochemical adaptations and defense responses in transgenic plants with perturbed polyamine metabolism

Marina Franceschetti, Barry Perry, Benjamin Thompson, Colin Hanfrey, Anthony J. Michael

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Soluble proteins from leaves of transgenic tobacco plants with perturbed polyamine metabolism, caused by S-adenosylmethionine decarboxylase overexpression, were analysed by comparative proteomics. A group of proteins was found to be increasingly repressed, in parallel with the degree of polyamine perturbation, in each of the three independent transgenic lines. These were identified as isoforms of chloroplast ribonucleoproteins, known to be involved in chloroplast mRNA stability, processing and translation. Another group of eight proteins strongly induced in the most metabolically perturbed line was identified as multiple, uncharacterised isoforms of the defense protein PR-1, a known marker for systemic acquired resistance.

Original languageEnglish (US)
Pages (from-to)477-480
Number of pages4
JournalFEBS Letters
Volume576
Issue number3
DOIs
StatePublished - Oct 22 2004

Keywords

  • Chloroplast ribonucleoprotein
  • PR-1
  • Polyamine
  • Proteomic
  • S-Adenosylmethionine decarboxylase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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