@inbook{dcf36300ceb941bab4ba73a694a9a353,
title = "Expression, purification, and analysis of recombinant drosophila dicer-1 and dicer-2 enzymes",
abstract = "RNA interference (RNAi) is a form of posttranscriptional gene silencing mediated by microRNA (miRNA) and small interfering RNA (siRNA). In Drosophila melanogaster, the RNase III enzymes Dicer-1 and Dicer-2 generate miRNA and siRNA, respectively. We describe the methods for the expression, purification, and analysis of recombinant Dicer-1 and Dicer-2 enzymes. Our studies demonstrate that Dicer-1 and Dicer-2 display different substrate specificities and ATP requirements.",
keywords = "Baculovirus, Dicer-1, Dicer-2, DsRNA, MiRNA, Pre-miRNA, RNA interference, SiRNA",
author = "Xuecheng Ye and Qinghua Liu",
year = "2008",
doi = "10.1007/978-1-59745-191-8_2",
language = "English (US)",
isbn = "9781588298744",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "11--27",
booktitle = "RNAi",
}