Expression, purification, and analysis of recombinant drosophila dicer-1 and dicer-2 enzymes

Xuecheng Ye, Qinghua Liu

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Citations (Scopus)

Abstract

RNA interference (RNAi) is a form of posttranscriptional gene silencing mediated by microRNA (miRNA) and small interfering RNA (siRNA). In Drosophila melanogaster, the RNase III enzymes Dicer-1 and Dicer-2 generate miRNA and siRNA, respectively. We describe the methods for the expression, purification, and analysis of recombinant Dicer-1 and Dicer-2 enzymes. Our studies demonstrate that Dicer-1 and Dicer-2 display different substrate specificities and ATP requirements.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages11-27
Number of pages17
Volume442
ISBN (Print)9781588298744
DOIs
StatePublished - 2008

Publication series

NameMethods in Molecular Biology
Volume442
ISSN (Print)10643745

Fingerprint

Ribonuclease III
Drosophila
RNA Interference
MicroRNAs
Small Interfering RNA
Substrate Specificity
Drosophila melanogaster
Adenosine Triphosphate

Keywords

  • Baculovirus
  • Dicer-1
  • Dicer-2
  • DsRNA
  • MiRNA
  • Pre-miRNA
  • RNA interference
  • SiRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ye, X., & Liu, Q. (2008). Expression, purification, and analysis of recombinant drosophila dicer-1 and dicer-2 enzymes. In Methods in Molecular Biology (Vol. 442, pp. 11-27). (Methods in Molecular Biology; Vol. 442). Humana Press. https://doi.org/10.1007/978-1-59745-191-8_2

Expression, purification, and analysis of recombinant drosophila dicer-1 and dicer-2 enzymes. / Ye, Xuecheng; Liu, Qinghua.

Methods in Molecular Biology. Vol. 442 Humana Press, 2008. p. 11-27 (Methods in Molecular Biology; Vol. 442).

Research output: Chapter in Book/Report/Conference proceedingChapter

Ye, X & Liu, Q 2008, Expression, purification, and analysis of recombinant drosophila dicer-1 and dicer-2 enzymes. in Methods in Molecular Biology. vol. 442, Methods in Molecular Biology, vol. 442, Humana Press, pp. 11-27. https://doi.org/10.1007/978-1-59745-191-8_2
Ye X, Liu Q. Expression, purification, and analysis of recombinant drosophila dicer-1 and dicer-2 enzymes. In Methods in Molecular Biology. Vol. 442. Humana Press. 2008. p. 11-27. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-191-8_2
Ye, Xuecheng ; Liu, Qinghua. / Expression, purification, and analysis of recombinant drosophila dicer-1 and dicer-2 enzymes. Methods in Molecular Biology. Vol. 442 Humana Press, 2008. pp. 11-27 (Methods in Molecular Biology).
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