Expression, purification, and analysis of recombinant drosophila dicer-1 and dicer-2 enzymes

Xuecheng Ye, Qinghua Liu

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Scopus citations

Abstract

RNA interference (RNAi) is a form of posttranscriptional gene silencing mediated by microRNA (miRNA) and small interfering RNA (siRNA). In Drosophila melanogaster, the RNase III enzymes Dicer-1 and Dicer-2 generate miRNA and siRNA, respectively. We describe the methods for the expression, purification, and analysis of recombinant Dicer-1 and Dicer-2 enzymes. Our studies demonstrate that Dicer-1 and Dicer-2 display different substrate specificities and ATP requirements.

Original languageEnglish (US)
Title of host publicationRNAi
Subtitle of host publicationDesign and Application
PublisherHumana Press
Pages11-27
Number of pages17
ISBN (Print)9781588298744
DOIs
StatePublished - 2008

Publication series

NameMethods in Molecular Biology
Volume442
ISSN (Print)1064-3745

Keywords

  • Baculovirus
  • Dicer-1
  • Dicer-2
  • DsRNA
  • MiRNA
  • Pre-miRNA
  • RNA interference
  • SiRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Ye, X., & Liu, Q. (2008). Expression, purification, and analysis of recombinant drosophila dicer-1 and dicer-2 enzymes. In RNAi: Design and Application (pp. 11-27). (Methods in Molecular Biology; Vol. 442). Humana Press. https://doi.org/10.1007/978-1-59745-191-8_2