Extended Lifetime In Vivo Pulse Stimulated Ultrasound Imaging

James Wang, Christopher V. Barback, Casey N. Ta, Joi Weeks, Natalie Gude, Robert F. Mattrey, Sarah L. Blair, William C. Trogler, Hotaik Lee, Andrew C. Kummel

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

An on-demand long-lived ultrasound contrast agent that can be activated with single pulse stimulated imaging (SPSI) has been developed using hard shell liquid perfluoropentane filled silica 500 nm nanoparticles for tumor ultrasound imaging. SPSI was tested on LnCAP prostate tumor models in mice; tumor localization was observed after intravenous (IV) injection of the contrast agent. Consistent with enhanced permeability and retention, the silica nanoparticles displayed an extended imaging lifetime of 3.3 ± 1 days (mean ± standard deviation). With added tumor specific folate functionalization, the useful lifetime was extended to 12 ±2 days; in contrast to ligand based tumor targeting, the effect of the ligands in this application is enhanced nanoparticle retention by the tumor. The current study demonstrates for the first time that IV injected functionalized silica contrast agents can be imaged with an in vivo lifetime ~500 times longer than current microbubble-based contrast agents. Such functionalized long-lived contrast agents may lead to new applications in tumor monitoring and therapy.

Original languageEnglish (US)
JournalIEEE Transactions on Medical Imaging
DOIs
StateAccepted/In press - Aug 17 2017

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Keywords

  • Contrast Agents
  • Imaging
  • In vivo
  • Liquids
  • Mice
  • Nanoparticles
  • Prostate
  • Silicon compounds
  • Tumors
  • Ultrasonic imaging
  • Ultrasound
  • Visualization

ASJC Scopus subject areas

  • Software
  • Radiological and Ultrasound Technology
  • Computer Science Applications
  • Electrical and Electronic Engineering

Cite this

Wang, J., Barback, C. V., Ta, C. N., Weeks, J., Gude, N., Mattrey, R. F., Blair, S. L., Trogler, W. C., Lee, H., & Kummel, A. C. (Accepted/In press). Extended Lifetime In Vivo Pulse Stimulated Ultrasound Imaging. IEEE Transactions on Medical Imaging. https://doi.org/10.1109/TMI.2017.2740784