External Qi of Yan Xin Qigong induces cell death and gene expression alterations promoting apoptosis and inhibiting proliferation, migration and glucose metabolism in small-cell lung cancer cells

Xin Yan, Feng Li, Igor Dozmorov, Mark Barton Frank, Ming Dao, Michael Centola, Wei Cao, Dan Hu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Small-cell lung cancer (SCLC) is a highly malignant carcinoma with poor long-term survival. Effective treatment remains highly demanded. In the present study, we demonstrated that External Qi of Yan Xin Qigong (YXQ-EQ) exerted potent cytotoxic effect towards SCLC cell line NCI-H82 via induction of apoptosis. Global gene expression profiling identified 39 genes whose expression was altered by YXQ-EQ in NCI-82 cells. Among them, semi-quantitative RT-PCR and real-time qPCR analyses confirmed that the gene expression levels of apoptotic proteins death-associated protein kinase 2 and cell death-inducing DFFA-like effector b were upregulated, whereas that of oncoproteins DEK and MYCL1, cell migration-promoting proteins CD24 and integrin-alpha 9, and glycolytic enzyme aldolase A were downregulated. These findings suggest that YXQ-EQ may exert anticancer effect through modulating gene expression in a way that facilitates cancer cell apoptosis while represses proliferation, metastasis, and glucose metabolism.

Original languageEnglish (US)
Pages (from-to)245-255
Number of pages11
JournalMolecular and Cellular Biochemistry
Volume363
Issue number1-2
DOIs
StatePublished - Apr 2012

Fingerprint

Qigong
Qi
Small Cell Lung Carcinoma
Cell death
Metabolism
Gene expression
Cell Death
Cells
Apoptosis
Gene Expression
Glucose
Death-Associated Protein Kinases
Fructose-Bisphosphate Aldolase
Oncogene Proteins
Gene Expression Profiling
Cell Movement
Real-Time Polymerase Chain Reaction
Proteins
Down-Regulation
Neoplasm Metastasis

Keywords

  • Anti-cancer effect
  • Cell death
  • Gene expression
  • Small-cell lung cancer

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

External Qi of Yan Xin Qigong induces cell death and gene expression alterations promoting apoptosis and inhibiting proliferation, migration and glucose metabolism in small-cell lung cancer cells. / Yan, Xin; Li, Feng; Dozmorov, Igor; Frank, Mark Barton; Dao, Ming; Centola, Michael; Cao, Wei; Hu, Dan.

In: Molecular and Cellular Biochemistry, Vol. 363, No. 1-2, 04.2012, p. 245-255.

Research output: Contribution to journalArticle

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AU - Li, Feng

AU - Dozmorov, Igor

AU - Frank, Mark Barton

AU - Dao, Ming

AU - Centola, Michael

AU - Cao, Wei

AU - Hu, Dan

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AB - Small-cell lung cancer (SCLC) is a highly malignant carcinoma with poor long-term survival. Effective treatment remains highly demanded. In the present study, we demonstrated that External Qi of Yan Xin Qigong (YXQ-EQ) exerted potent cytotoxic effect towards SCLC cell line NCI-H82 via induction of apoptosis. Global gene expression profiling identified 39 genes whose expression was altered by YXQ-EQ in NCI-82 cells. Among them, semi-quantitative RT-PCR and real-time qPCR analyses confirmed that the gene expression levels of apoptotic proteins death-associated protein kinase 2 and cell death-inducing DFFA-like effector b were upregulated, whereas that of oncoproteins DEK and MYCL1, cell migration-promoting proteins CD24 and integrin-alpha 9, and glycolytic enzyme aldolase A were downregulated. These findings suggest that YXQ-EQ may exert anticancer effect through modulating gene expression in a way that facilitates cancer cell apoptosis while represses proliferation, metastasis, and glucose metabolism.

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