TY - JOUR
T1 - Extracellular signal-regulated kinases in T cells
T2 - Characterization of human ERK1 and ERK2 cDNAs
AU - Owaki, Hajime
AU - Makar, Robert
AU - Boulton, Teri G.
AU - Cobb, Melanie H.
AU - Geppert, Thomas D.
N1 - Funding Information:
Public Health Services grants AR09989, AR39169 from the American Cancer Society. Dr. Cobb is Development Award from the NM.
Funding Information:
1 This work was supported by U.S. and DK34128 and a grant-in-aid a recipient of a Research Career
PY - 1992/2/14
Y1 - 1992/2/14
N2 - Extracellular signal-regulated kinases 1 and 2 are growth factor-sensitive serine/threonine kinases. cDNAs for both human kinases were isolated and sequenced. The nucleic acid and deduced protein sequences of human extracellular signal-regulated kinase 1 were 88% and 96% identical, respectively, to the homologous rat sequences. The nucleic acid and deduced protein sequences of human extracellular signal-regulated kinase 2 were 90% and 98% identical, respectively, to the corresponding rat sequences. A human extracellular signal-regulated kinase 2 specific probe was used to demonstrate that the mRNA for this kinase was present in T cells and did not change with activation. The deduced protein sequences of both human kinases were greater than 95% identical to two Xenopus kinase sequences, indicating that these enzymes are highly conserved across species.
AB - Extracellular signal-regulated kinases 1 and 2 are growth factor-sensitive serine/threonine kinases. cDNAs for both human kinases were isolated and sequenced. The nucleic acid and deduced protein sequences of human extracellular signal-regulated kinase 1 were 88% and 96% identical, respectively, to the homologous rat sequences. The nucleic acid and deduced protein sequences of human extracellular signal-regulated kinase 2 were 90% and 98% identical, respectively, to the corresponding rat sequences. A human extracellular signal-regulated kinase 2 specific probe was used to demonstrate that the mRNA for this kinase was present in T cells and did not change with activation. The deduced protein sequences of both human kinases were greater than 95% identical to two Xenopus kinase sequences, indicating that these enzymes are highly conserved across species.
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U2 - 10.1016/0006-291X(92)91891-S
DO - 10.1016/0006-291X(92)91891-S
M3 - Article
C2 - 1540184
AN - SCOPUS:0026578183
SN - 0006-291X
VL - 182
SP - 1416
EP - 1422
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -