Extraction and quantification of daunomycin and doxorubicin in tissues

J. F. Strauss, R. L. Kitchens, V. W. Patrizi, E. P. Frenkel

Research output: Chapter in Book/Report/Conference proceedingChapter

21 Scopus citations

Abstract

The measurement of intracellular concentrations of the anti-cancer drug doxorubicin was performed by the application of a simple cell extraction technique combined with a rapid high-performance liquid chromatographic separation. Quantitation was done by fluorescence detection. The extraction procedure was non-degradative and the mean recovery of drug was 95%. A high drug extraction efficiency was confirmed with radiolabeled [3H]doxorubicin. The method is applicable to normal and neoplastic tissue.

Original languageEnglish (US)
Title of host publicationJournal of Chromatography
Pages139-144
Number of pages6
Volume221
Edition1
StatePublished - 1980

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Medicine

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    Strauss, J. F., Kitchens, R. L., Patrizi, V. W., & Frenkel, E. P. (1980). Extraction and quantification of daunomycin and doxorubicin in tissues. In Journal of Chromatography (1 ed., Vol. 221, pp. 139-144)