The measurement of intracellular concentrations of the anti-cancer drug doxorubicin was performed by the application of a simple cell extraction technique combined with a rapid high-performance liquid chromatographic separation. Quantitation was done by fluorescence detection. The extraction procedure was non-degradative and the mean recovery of drug was 95%. A high drug extraction efficiency was confirmed with radiolabeled [3H]doxorubicin. The method is applicable to normal and neoplastic tissue.
|Original language||English (US)|
|Title of host publication||Journal of Chromatography|
|Number of pages||6|
|State||Published - 1980|
ASJC Scopus subject areas
- Clinical Biochemistry
- Molecular Medicine