Extraction of cellular DNA from human cells and tissues fixed in ethanol

Linda J. Smith, Raul C. Braylan, Judith E. Nutkis, Kathryn B. Edmundson, James R. Downing, Edward K. Wakeland

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

DNA can be extracted from ethanol-fixed lymphoid cells and tissues. The fixation procedure is simple and rapid, and the DNA extraction itself is the same as that normally used for fresh tissue or cells. DNA extracted from ethanol-fixed material is indistinguishable from DNA extracted from fresh samples based on its purity, its ability to be digested with restriction endonucleases, and its ability to specifically hybridize to DNA probes. The capability to extract DNA from ethanol-fixed cells and tissues eliminates the need for stringent handling and storage requirements of fresh or frozen specimens.

Original languageEnglish (US)
Pages (from-to)135-138
Number of pages4
JournalAnalytical biochemistry
Volume160
Issue number1
DOIs
StatePublished - Jan 1987

Keywords

  • cell preservation
  • cellular DNA
  • human lymphoma
  • lymphocytes
  • restriction enzyme

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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