Fabrication of resorbable microporous intravascular stents for gene therapy applications

G. Rajasubramanian, R. S. Meidell, C. Landau, M. L. Dollar, D. B. Holt, J. E. Willard, M. D. Prager, R. C. Eberhart

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

The authors have produced resorbable, microporous endoluminal stents from Poly-L-lactic acid (PLLA)/Poly ε-caprolactone (PCL) blends. Both helical and tube stent designs have been obtained by solvent casting and flotation-precipitation fabrication techniques. A range of PLLA/PCL blend ratios and process variables were employed to investigate their influence on mechanical properties, porosity, and degradation rate. Polymer blends with higher PLLA proportions exhibit higher elastic moduli and ultimate tensile strength, and lower elongation, porosity, and degradation rates than do materials with higher PCL content. Stents with suitable mechanical properties for deployment and support of the vessel wall were obtained. Poly(ethylene oxide) was incorporated into these devices using an acid swelling technique, opening the pore structure and improving the hydrophilic character, thereby enabling the uptake of recombinant adenoviral vectors. The 50:50 PLLA/PCL blended stents were impregnated with recombinant adenovirus (AdCMBβGal, encoding a nuclear localizing variant of Escherichia coli β-galactosidase). Cultured CV-1 cells incubated with stents impregnated with the recombinant virus expressed nuclear localized β-galactosidase activity, confirming that absorbed virus is released from the matrix in an infectious form, with kinetics suggesting that genetically enhanced endovascular devices of this design are feasible.

Original languageEnglish (US)
Pages (from-to)M584-M589
JournalASAIO Journal
Volume40
Issue number3
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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    Rajasubramanian, G., Meidell, R. S., Landau, C., Dollar, M. L., Holt, D. B., Willard, J. E., Prager, M. D., & Eberhart, R. C. (1994). Fabrication of resorbable microporous intravascular stents for gene therapy applications. ASAIO Journal, 40(3), M584-M589. https://doi.org/10.1097/00002480-199407000-00066