FACS purification of bone marrow-derived epidermal populations in mice: Langerhans cells and Thy-1+ dendritic cells

S. Sullivan, P. R. Bergstresser, R. E. Tigelaar, J. W. Streilein

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

A method was developed which allows for the separation and purification of Langerhans cells (LC) and Thy-1+ cells (Thy-1+dEC) from mouse epidermis. Epidermal cell (EC) suspensions were subjected to Ficoll separation, and the resulting interface EC were harvested. These EC were then 'tagged' with the appropriate monoclonal antibody and sorted into positive and negative populations using the Fluorescence Activated Cell Sorter (FACS). Preparations of viable LC and Thy1+dEC were obtained with 94-98% and 94-99% purities, respectively.

Original languageEnglish (US)
Pages (from-to)491-495
Number of pages5
JournalJournal of Investigative Dermatology
Volume84
Issue number6
DOIs
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Fingerprint Dive into the research topics of 'FACS purification of bone marrow-derived epidermal populations in mice: Langerhans cells and Thy-1<sup>+</sup> dendritic cells'. Together they form a unique fingerprint.

  • Cite this