Fast 18F labeling of a near-infrared fluorophore enables positron emission tomography and optical imaging of sentinel lymph nodes

Richard Ting, Todd A. Aguilera, Jessica L. Crisp, David J. Hall, William C. Eckelman, David R. Vera, Roger Y. Tsien

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

We combine a novel boronate trap for F- with a near-infrared fluorophore into a single molecule. Attachment to targeting ligands enables localization by positron emission tomography (PET) and near-infrared fluorescence (NIRF). Our first application of this generic tag is to label Lymphoseek (tilmanocept), an agent designed for receptor-specific sentinel lymph node (SLN) mapping. The new conjugate incorporates 18F- in a single, aqueous step, targets mouse SLN rapidly (1 h) with reduced distal lymph node accumulation, permits PET or scintigraphic imaging of SLN, and enables NIRF-guided excision and histological verification even after 18F decay. This embodiment is superior to current SLN mapping agents such as nontargeted [99mTc]sulfur colloids and Isosulfan Blue, as well as the phase III targeted ligand [99mTc]SPECT Lymphoseek counterpart, species that are visible by SPECT or visible absorbance separately. Facile incorporation of 18F into a NIRF probe should promote many synergistic PET and NIRF combinations.

Original languageEnglish (US)
Pages (from-to)1811-1819
Number of pages9
JournalBioconjugate Chemistry
Volume21
Issue number10
DOIs
StatePublished - Oct 20 2010

Fingerprint

Positron emission tomography
Fluorophores
Optical Imaging
Positron-Emission Tomography
Labeling
Fluorescence
Infrared radiation
Imaging techniques
Single-Photon Emission-Computed Tomography
Ligands
Colloids
Sulfur
Lymph Nodes
Labels
cyhalothrin
Sentinel Lymph Node
Molecules
technetium-diethylenetriaminepentaacetic acid-mannosyl-dextran

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Organic Chemistry
  • Pharmaceutical Science
  • Biomedical Engineering
  • Pharmacology

Cite this

Fast 18F labeling of a near-infrared fluorophore enables positron emission tomography and optical imaging of sentinel lymph nodes. / Ting, Richard; Aguilera, Todd A.; Crisp, Jessica L.; Hall, David J.; Eckelman, William C.; Vera, David R.; Tsien, Roger Y.

In: Bioconjugate Chemistry, Vol. 21, No. 10, 20.10.2010, p. 1811-1819.

Research output: Contribution to journalArticle

Ting, Richard ; Aguilera, Todd A. ; Crisp, Jessica L. ; Hall, David J. ; Eckelman, William C. ; Vera, David R. ; Tsien, Roger Y. / Fast 18F labeling of a near-infrared fluorophore enables positron emission tomography and optical imaging of sentinel lymph nodes. In: Bioconjugate Chemistry. 2010 ; Vol. 21, No. 10. pp. 1811-1819.
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AU - Aguilera, Todd A.

AU - Crisp, Jessica L.

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AU - Eckelman, William C.

AU - Vera, David R.

AU - Tsien, Roger Y.

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