Fat2 acts through the WAVE regulatory complex to drive collective cell migration during tissue rotation

Anna Julia Squarr, Klaus Brinkmann, Baoyu Chen, Tim Steinbacher, Klaus Ebnet, Michael K. Rosen, Sven Bogdan

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Directional cell movements during morphogenesis require the coordinated interplay between membrane receptors and the actin cytoskeleton. The WAVE regulatory complex (WRC) is a conserved actin regulator. Here, we found that the atypical cadherin Fat2 recruits the WRC to basal membranes of tricellular contacts where a new type of planar-polarized whip-like actin protrusion is formed. Loss of either Fat2 function or its interaction with the WRC disrupts tricellular protrusions and results in the formation of nonpolarized filopodia. We provide further evidence for a molecular network in which the receptor tyrosine phosphatase Dlar interacts with the WRC to couple the extracellular matrix, the membrane, and the actin cytoskeleton during egg elongation. Our data uncover a mechanism by which polarity information can be transduced from a membrane receptor to a key actin regulator to control collective follicle cell migration during egg elongation. 4D-live imaging of rotating MCF10A mammary acini further suggests an evolutionary conserved mechanism driving rotational motions in epithelial morphogenesis.

Original languageEnglish (US)
Pages (from-to)591-603
Number of pages13
JournalJournal of Cell Biology
Volume212
Issue number5
DOIs
StatePublished - 2016

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Cell Movement
Actins
Membranes
Actin Cytoskeleton
Morphogenesis
Ovum
Pseudopodia
Cadherins
Extracellular Matrix
Breast

ASJC Scopus subject areas

  • Cell Biology

Cite this

Fat2 acts through the WAVE regulatory complex to drive collective cell migration during tissue rotation. / Squarr, Anna Julia; Brinkmann, Klaus; Chen, Baoyu; Steinbacher, Tim; Ebnet, Klaus; Rosen, Michael K.; Bogdan, Sven.

In: Journal of Cell Biology, Vol. 212, No. 5, 2016, p. 591-603.

Research output: Contribution to journalArticle

Squarr, Anna Julia ; Brinkmann, Klaus ; Chen, Baoyu ; Steinbacher, Tim ; Ebnet, Klaus ; Rosen, Michael K. ; Bogdan, Sven. / Fat2 acts through the WAVE regulatory complex to drive collective cell migration during tissue rotation. In: Journal of Cell Biology. 2016 ; Vol. 212, No. 5. pp. 591-603.
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