Features of influenza HA required for apical sorting differ from those required for association with DRMS or MAL

Renee D. Tall, Miguel A. Alonso, Michael G. Roth

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The influenza virus hemagglutinin (HA) is sorted to the apical membrane in polarized epithelial cells and associates with detergent-resistant membranes (DRMs). By systematic mutagenesis of the transmembrane residues, we show that hemagglutinin requires 10 contiguous transmembrane amino acids to enter detergent-resistant membranes and that the surface of the trimeric hemagglutinin transmembrane domain facing the lipid environment as well as that facing the interior of the trimer is important for stable association with detergent-resistant membranes. However, association with detergent-resistant membranes was not required for apical sorting. MAL/VIP17 is a protein that is required for apical transport and a small fraction of hemagglutinin co-precipitates with MAL. Mutations that prevented HA from being isolated in detergent-resistant membranes decreased co-precipitation with MAL. The hemagglutinin and MAL that co-precipitated were contained in a detergent-resistant vesicle. However, most of the co-precipitation of newly synthesized hemagglutinin with MAL occurred only after the majority of hemagglutinin reached the cell surface. Both the timing and the limited extent of co-precipitation suggest that the majority of vesicles containing hemagglutinin and MAL are not the detergent-resistant membrane transport intermediates carrying hemagglutinin from the TGN to the apical surface.

Original languageEnglish (US)
Pages (from-to)838-849
Number of pages12
JournalTraffic
Volume4
Issue number12
DOIs
StatePublished - Dec 2003

Fingerprint

Hemagglutinins
Sorting
Human Influenza
Detergents
Membranes
Coprecipitation
Mutagenesis
Orthomyxoviridae
Viruses
Precipitates
Epithelial Cells
Lipids
Amino Acids
Mutation

Keywords

  • Apical sorting
  • HA
  • Lipid raft
  • MAL
  • Sorting signals

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Structural Biology
  • Molecular Biology
  • Genetics

Cite this

Features of influenza HA required for apical sorting differ from those required for association with DRMS or MAL. / Tall, Renee D.; Alonso, Miguel A.; Roth, Michael G.

In: Traffic, Vol. 4, No. 12, 12.2003, p. 838-849.

Research output: Contribution to journalArticle

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