Fecal corticosterone levels in RCAN1 mutant mice

Tammy Rakowski-Anderson, Helen Wong, Beverly Rothermel, Peter Cain, Carmencita Lavilla, Jennifer K. Pullium, Charles Hoeffer

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Regulator of calcineurin 1 (RCAN1) is related to the expression of human neurologic disorders such as Down syndrome, Alzheimer disease, and chromosome 21q deletion syndrome. We showed here that RCAN1-knockout mice exhibit reduced innate anxiety as indicated by the elevated-plus maze. To examine whether glucocorticoids contribute to this phenotype, we measured fecal corticosterone in male wildtype and RCAN1-knockout mice and in male and female transgenic mice with neuronal overexpression of RCAN1 (Tg-RCAN1TG). We found no difference in fecal corticosterone levels of RCAN1-knockout mice and their wildtype littermates. As expected, we found differences between sexes in fecal corticosterone levels. In addition, we found higher levels of excreted corticosterone in Tg-RCAN1TG female mice as compared with female wildtype mice. Our data indicate normal diurnal corticosterone production in RCAN1 mutant mice and do not suggest a causal role in either the cognitive or anxiety phenotypes exhibited by RCAN1-knockout mice.

Original languageEnglish (US)
Pages (from-to)87-94
Number of pages8
JournalComparative Medicine
Volume62
Issue number2
StatePublished - Apr 2012

Fingerprint

Calcineurin
corticosterone
Corticosterone
mutants
Knockout Mice
mice
anxiety
Anxiety
Phenotype
Chromosome Deletion
Down syndrome
phenotype
chromosome elimination
Chromosomes
nervous system diseases
Down Syndrome
Nervous System Diseases
Sex Characteristics
Transgenic Mice
Glucocorticoids

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

Rakowski-Anderson, T., Wong, H., Rothermel, B., Cain, P., Lavilla, C., Pullium, J. K., & Hoeffer, C. (2012). Fecal corticosterone levels in RCAN1 mutant mice. Comparative Medicine, 62(2), 87-94.

Fecal corticosterone levels in RCAN1 mutant mice. / Rakowski-Anderson, Tammy; Wong, Helen; Rothermel, Beverly; Cain, Peter; Lavilla, Carmencita; Pullium, Jennifer K.; Hoeffer, Charles.

In: Comparative Medicine, Vol. 62, No. 2, 04.2012, p. 87-94.

Research output: Contribution to journalArticle

Rakowski-Anderson, T, Wong, H, Rothermel, B, Cain, P, Lavilla, C, Pullium, JK & Hoeffer, C 2012, 'Fecal corticosterone levels in RCAN1 mutant mice', Comparative Medicine, vol. 62, no. 2, pp. 87-94.
Rakowski-Anderson T, Wong H, Rothermel B, Cain P, Lavilla C, Pullium JK et al. Fecal corticosterone levels in RCAN1 mutant mice. Comparative Medicine. 2012 Apr;62(2):87-94.
Rakowski-Anderson, Tammy ; Wong, Helen ; Rothermel, Beverly ; Cain, Peter ; Lavilla, Carmencita ; Pullium, Jennifer K. ; Hoeffer, Charles. / Fecal corticosterone levels in RCAN1 mutant mice. In: Comparative Medicine. 2012 ; Vol. 62, No. 2. pp. 87-94.
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