TY - JOUR
T1 - Fibroblast adhesion to fibrinogen and fibrin substrata
T2 - Requirement for cold-insoluble globulin (plasma fibronectin)
AU - Grinnell, Frederick
AU - Feld, Marian
AU - Minter, Dianne
N1 - Funding Information:
We are indebted to Dr. Deane Mosher for his helpful discussions during the course of these experiments and to Drs. Richard Anderson and William Snell for their comments during preparation of the manuscript. This research was supported by a grant from the NIH.
PY - 1980/2
Y1 - 1980/2
N2 - We carried out experiments to determine conditions for fibroblast adhesion to fibrinogen and fibrin substrata. Baby hamster kidney (BHK) cells did not attach to substrata composed of purified fibrinogen or fibrin. When cold-insoluble globulin (CIG) (plasma fibronectin) was bound to fibrinogen or fibrin substrata, adhesion of BHK cells was observed and the extent of adhesion was dependent upon the CIG concentration. Binding of CIG to fibrinogen or fibrin substrata in the presence of Factor XIII (fibrin-stabilizing factor) under covalent crosslinking conditions resulted in a marked increase in the ability of the substrata to support cell adhesion. Control experiments indicated that CIG formed the sites on the fibrinogen and fibrin substrata to which the cells were attaching. In addition, the effect of factor XIII was shown to require covalent crosslinking of CIG to the fibrinogen or fibrin, which involved a glutamine residue on the CIG molecule and could be prevented by prior crosslinking of CIG with putrescine or with itself. The enhanced ability of Factor XIII-crosslinked CIG substrata to support cell adhesion could not be accounted for by the absolute amount of CIG bound to the substrata. We present in this paper the possibility that the orientation of CIG on the substrata is the critical factor.
AB - We carried out experiments to determine conditions for fibroblast adhesion to fibrinogen and fibrin substrata. Baby hamster kidney (BHK) cells did not attach to substrata composed of purified fibrinogen or fibrin. When cold-insoluble globulin (CIG) (plasma fibronectin) was bound to fibrinogen or fibrin substrata, adhesion of BHK cells was observed and the extent of adhesion was dependent upon the CIG concentration. Binding of CIG to fibrinogen or fibrin substrata in the presence of Factor XIII (fibrin-stabilizing factor) under covalent crosslinking conditions resulted in a marked increase in the ability of the substrata to support cell adhesion. Control experiments indicated that CIG formed the sites on the fibrinogen and fibrin substrata to which the cells were attaching. In addition, the effect of factor XIII was shown to require covalent crosslinking of CIG to the fibrinogen or fibrin, which involved a glutamine residue on the CIG molecule and could be prevented by prior crosslinking of CIG with putrescine or with itself. The enhanced ability of Factor XIII-crosslinked CIG substrata to support cell adhesion could not be accounted for by the absolute amount of CIG bound to the substrata. We present in this paper the possibility that the orientation of CIG on the substrata is the critical factor.
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U2 - 10.1016/0092-8674(80)90526-7
DO - 10.1016/0092-8674(80)90526-7
M3 - Article
C2 - 7357618
AN - SCOPUS:0018838451
SN - 0092-8674
VL - 19
SP - 517
EP - 525
JO - Cell
JF - Cell
IS - 2
ER -